Kang, HC Kim, S Lee, M Bae, YH 2024-01-21T04:42:28Z 2024-01-21T04:42:28Z 2021-09-01 2005-06-20 0168-3659 https://pubs.kist.re.kr/handle/201004/136360 Ex vivo transfer of therapeutic genes to cells is one of the potential strategies to prolong the life span of cell transplants. However, relatively safe non-viral carriers have not been extensively investigated due to their lower transfection efficiency. In this study, poly(L-lysine)-g-sulfonylurea varying SU content (PLL-SU) was synthesized to promote gene delivery efficacy to an insulin secreting cell line, RINm5F, which is known to express sulfonylurea receptor (SUR). The polymer formed complexes with a model reporter gene of pCMV-Luc (DNA) and the size of resulting particles was around 100 nm. The transfection efficiency of a polymer synthesized with 5 mol% of SU in the reaction feed (PLL-SU5%) to RINm5F cell was at least 5 times higher than that of PLL. The cytotoxicity of PLL-SU5%/DNA complex was equivalent to that of PLL/DNA complex. PLL-SU5% showed less transfection efficiency than PLL to NIH3T3 and HepG2 cells which are SUR negative. In RINm5F cells, the addition of free SU decreased the transfection efficiency of PLL-SU5%/DNA complex, suggesting that the complex shares the same receptors for SU. The PLL-SU5%/DNA complex seems to be internalized via SUR-mediated endocytosis pathway as suggested by vacuolar ATPases inhibition by Bafilomycin A(t). It is noted that RINm5F cells treated with PLL-SU5%/DNA complex secreted more insulin than control, untreated cells, suggesting the insulinotropic effect of SU in PLL-SU5%. In conclusion, PLL-SU may be useful for transfer of therapeutic genes into insulin secreting cells. (c) 2005 Elsevier B.V. All rights reserved. English ELSEVIER SCIENCE BV HUMAN PANCREATIC-ISLETS IN-VITRO CATECHOLAMINE SECRETION SULFONYLUREA RECEPTOR DELIVERY-SYSTEMS NERVOUS-SYSTEM VIRAL VECTORS VIVO THERAPY DNA Polymeric gene carrier for insulin secreting cells: Poly(L-lysine)-g-sulfonylurea for receptor mediated transfection Article 10.1016/j.jconrel.2005.03.013 1 JOURNAL OF CONTROLLED RELEASE, v.105, no.1-2, pp.164 - 176 JOURNAL OF CONTROLLED RELEASE 105 1-2 164 176 scie scopus 000230576600015 2-s2.0-20444401547 Chemistry, Multidisciplinary Pharmacology & Pharmacy Chemistry Pharmacology & Pharmacy Article HUMAN PANCREATIC-ISLETS IN-VITRO CATECHOLAMINE SECRETION SULFONYLUREA RECEPTOR DELIVERY-SYSTEMS NERVOUS-SYSTEM VIRAL VECTORS VIVO THERAPY DNA gene delivery receptor-mediated endocytosis polymeric non-viral vector sulfonylurea insulin-secreting cells