Jeong, KS Lee, J Jeong, W Noh, DH Do, SH Kim, YK 2024-01-21T05:06:17Z 2024-01-21T05:06:17Z 2021-09-03 2005-05 0171-967X https://pubs.kist.re.kr/handle/201004/136512 Estrogen loss has been known to increase bone turnover through accelerated bone resorption coupled by increased bone formation. In the present study, we measured estrogen effect on bone turnover by incorporation of H-2 from (H2O)-H-2 into amino acids. At 6 weeks of age, rats were either sham-operated (sham) or ovariectornized (ovx). Two weeks after surgery, 17 beta-estradiol (est) was implanted subcutaneously to ovx rats. At 9 weeks of age, (H2O)-H-2 labeling started by administration of 4% (H2O)-H-2 to rats for 4 or 7 weeks in drinking water after a single intraperitonial bolus injection with 99.9% (H2O)-H-2. Body (H2O)-H-2 enrichments were stable at approximately 3.0% over labeling period. Fractional replacements (f) of the midshaft femur were higher in the sham group (40.36 +/- 4.89% vs 42.47 +/- 11.22%) than the ovx (28.57 +/- 9.67% vs 37.47 +/- 8.34%) and est (26.57 +/- 4.00% vs 30.35 +/- 5.34%) groups 4 and 7 weeks after labeling, respectively. Ovariectomy-induced bone loss was observed in the trabecular bone along with a significantly increased number of osteoclasts, all of which were normalized after estradiol treatment. Taken together, our results indicate that estrogen deficiency significantly reduces the proportion of newly synthesized bone matrix as well as the total amount of bone matrix. The reduced portion of new matrix in ovx rats, presumably caused by activated osteoclastic degradation, was compensated rapidly with time. In addition, estradiol treatment protected the bone matrix by decreasing bone turnover rate. English SPRINGER IN-VIVO OSTEOPOROSIS MARKERS RATES Measurement of estrogen effect on bone turnover by (H2O)-H-2 labeling Article 10.1007/s00223-004-1103-z 1 CALCIFIED TISSUE INTERNATIONAL, v.76, no.5, pp.365 - 370 CALCIFIED TISSUE INTERNATIONAL 76 5 365 370 scie scopus 000230091700007 2-s2.0-23944465492 Endocrinology & Metabolism Endocrinology & Metabolism Article IN-VIVO OSTEOPOROSIS MARKERS RATES estrogen ovariectomy bone matrix (H2O)-H-2 bone turnover