Cho, SW Park, HJ Ryu, JH Kim, SH Kim, YH Choi, CY Lee, MJ Kim, JS Jang, IS Kim, DI Kim, BS 2024-01-21T05:06:23Z 2024-01-21T05:06:23Z 2021-09-03 2005-05 0142-9612 https://pubs.kist.re.kr/handle/201004/136514 Synthetic polymer vascular patches used in cardiovascular surgery have shortcomings such as thrombosis. intimal hyperplasia, calcification, infection, and no growth potential. Tissue-engineered vascular patches using autologous vascular cells may solve these problems. In this study, we developed a tissue-engineered vascular patch using autologous bone marrow-derived cells (BMCs) and decellularized tissue matrices. Vascular smooth muscle cells and endothelial cells were differentiated from bone mar-row mononuclear cells in vitro. Tissue-engineered vascular patches were fabricated by seeding these cells onto decellularized canine inferior vena cava matrices and implanted into the inferior vena cava of dogs. Three weeks after implantation. the tissue-engineered vascular patches were patent with no sign of thrombus formation. Histological, immunohistochemical, and electron microscopic analyses of the vascular patches retrieved 3 weeks after implantation revealed regeneration of endothelium and smooth muscle and the presence of collagen and elastin. BMCs labeled with a fluorescent dye prior to implantation were detected in the retrieved vascular patches, indicating that the BMCs survived after implantation and contributed to the vascular tissue regeneration. This study demonstrates that vascular patches can be tissue-engineered with autologous BMCs and decellularized tissue matrices. (C) 2004 Elsevier Ltd. All rights reserved. English ELSEVIER SCI LTD ENDOTHELIAL PROGENITOR CELLS CAROTID-ENDARTERECTOMY EXTRACELLULAR-MATRIX STROMAL CELLS IN-VIVO AUTOGRAFTS POLYTETRAFLUOROETHYLENE NEOVASCULARIZATION DIFFERENTIATION IMMUNOGENICITY Vascular patches tissue-engineered with autologous bone marrow-derived cells and decellularized tissue matrices Article 10.1016/j.biomaterials.2004.06.018 1 BIOMATERIALS, v.26, no.14, pp.1915 - 1924 BIOMATERIALS 26 14 1915 1924 scie scopus 000226663800032 2-s2.0-20844432521 Engineering, Biomedical Materials Science, Biomaterials Engineering Materials Science Article ENDOTHELIAL PROGENITOR CELLS CAROTID-ENDARTERECTOMY EXTRACELLULAR-MATRIX STROMAL CELLS IN-VIVO AUTOGRAFTS POLYTETRAFLUOROETHYLENE NEOVASCULARIZATION DIFFERENTIATION IMMUNOGENICITY vascular patch bone marrow-derived cell decellularized tissue matrix tissue engineering