Wee, KW Kang, GY Park, J Kang, JY Yoon, DS Park, JH Kim, TS 2024-01-21T05:07:47Z 2024-01-21T05:07:47Z 2021-09-03 2005-04-15 0956-5663 https://pubs.kist.re.kr/handle/201004/136540 We report an electro-mechanical biosensor for electrical detection of proteins with disease markers using self-sensing piezoresistive microcantilevers. Electrical detection, via surface stress changes, of antigen-antibody (Ag-Ab) specific binding was accomplished through a direct nano-mechanical response of micro-fabricated self-sensing micro-cantilevers. A piezoresistive sensor measures the film resistance variation with respect to surface stress caused by biomolecules specific binding. When specific binding occurred on a functionalized. An surface, surface stress was induced throughout the cantilever, resulting in cantilever bending and resistance change of the piezoresistive layer. The cantilever biosensors were used for the detection of prostate specific antigen (PSA) and C-reactive proteins (CRP), which are a specific marker of prostate cancer and cardiac disease. From the above experiment, it was revealed that the sensor output voltage was proportional to the injected antigen concentration (without antigen, 10 ng/ml, 100 ng/ml, 1 mu g/ml). PSA and CRP antibodies were found to be very specific for their antigens, respectively. This indicated that the self-sensing micro-cantilever approach is beneficial for detecting disease markers, and our piezoresistive micro-cantilever sensor system is applicable to miniaturized biosensor systems. (c) 2004 Elsevier B.V. All rights reserved. English ELSEVIER ADVANCED TECHNOLOGY C-REACTIVE PROTEIN ACUTE-PHASE RESPONSE READ-OUT SENSORS BIOSENSOR NOISE IONS Novel electrical detection of label-free disease marker proteins using piezoresistive self-sensing micro-cantilevers Article 10.1016/j.bios.2004.09.023 1 BIOSENSORS & BIOELECTRONICS, v.20, no.10, pp.1932 - 1938 BIOSENSORS & BIOELECTRONICS 20 10 1932 1938 scie scopus 000227845700006 2-s2.0-14644415474 Biophysics Biotechnology & Applied Microbiology Chemistry, Analytical Electrochemistry Nanoscience & Nanotechnology Biophysics Biotechnology & Applied Microbiology Chemistry Electrochemistry Science & Technology - Other Topics Article; Proceedings Paper C-REACTIVE PROTEIN ACUTE-PHASE RESPONSE READ-OUT SENSORS BIOSENSOR NOISE IONS micro-cantilever piezoresistivity nano-mechanics antigen antibody