Jeong, SI Kwon, JH Lim, JI Cho, SW Jung, YM Sung, WJ Kim, SH Kim, YH Lee, YM Kim, BS Choi, CY Kim, SJ 2024-01-21T05:11:21Z 2024-01-21T05:11:21Z 2021-09-03 2005-04 0142-9612 https://pubs.kist.re.kr/handle/201004/136606 Blood vessels are subjected in vivo to mechanical forces in a form of radial distention, encompassing cyclic mechanical strain due to the pulsatile nature of blood flow. Vascular smooth muscle (VSM) tissues engineered in vitro with a conventional tissue engineering technique may not be functional, because vascular smooth muscle cells (VSMCs) cultured in vitro typically revert from a contractile phenotype to a synthetic phenotype. In this study, we hypothesized that pulsatile strain and shear stress stimulate VSM tissue development and induce VSMCs to retain the differentiated phenotype in VSM engineering in vitro. To test the hypothesis, rabbit aortic smooth muscle cells (SMCs) were seeded onto rubber-like elastic, three-dimensional PLCL [poly(lactide-co-caprolactone), 50:50] scaffolds and subjected to pulsatile strain and shear stress by culturing them in pulsatile perfusion bioreactors for up to 8 weeks. As control experiments, VSMCs were cultured on PLCL scaffolds statically. The pulsatile strain and shear stress enhanced the VSMCs proliferation and collagen production. In addition, a significant cell alignment in a direction radial to the distending direction was observed in VSM tissues exposed to radial distention, which is similar to that of native VSM tissues in vivo, whereas VSMs in VSM tissues engineered in the static condition randomly aligned. Importantly, the expression of SM alpha-actin, a differentiated phenotype of SMCs, was upregulated by 2.5-fold in VSM tissues engineered under the mechano-active condition, compared to VSM tissues engineered in the static condition. This study demonstrates that tissue engineering of VSM tissues in vitro by using pulsatile perfusion bioreactors and elastic PLCL scaffolds leads to the enhancement of tissue development and the retention of differentiated cell phenotype. (C) 2004 Elsevier Ltd. All rights reserved. English ELSEVIER SCI LTD CELLS CULTURE PROLIFERATION ARTERIES BEHAVIOR Mechano-active tissue engineering of vascular smooth muscle using pulsatile perfusion bioreactors and elastic PLCL scaffolds Article 10.1016/j.biomaterials.2004.04.036 1 BIOMATERIALS, v.26, no.12, pp.1405 - 1411 BIOMATERIALS 26 12 1405 1411 scie scopus 000225760100009 2-s2.0-5044226134 Engineering, Biomedical Materials Science, Biomaterials Engineering Materials Science Article CELLS CULTURE PROLIFERATION ARTERIES BEHAVIOR vascular tissue engineering pulsatile perfusion bioreactor elastic poly-(L-lactide-co-epsilon-caprolactone) scaffold smooth muscle cell