Yoo, HS Lee, JE Chung, H Kwon, IC Jeong, SY 2024-01-21T05:13:45Z 2024-01-21T05:13:45Z 2021-09-03 2005-03-02 0168-3659 https://pubs.kist.re.kr/handle/201004/136651 A self-assembled nanoparticle was prepared using a hydrophobically modified glycol chitosan for gene delivery. A primary amine of glycol chitosan was modified with 5 beta-cholanic acid to prepare a hydrophobically modified glycol chitosan (HGC). The modified chitosan spontaneously formed DNA nanoparticles by a hydrophobic interaction between HGC and hydrophobized DNA. As the HGC content increased, the encapsulation efficiencies of DNA increased while the size of HGC nanoparticles decreased. Upon increasing HGC contents, HGC nanoparticle became less cytotoxic. The increased HGC contents also facilitated endocytic uptakes of HGC nanoparticles by COS-1 cells, which were confirmed by a confocal microscopy. The HGC nanoparticles showed increasing in vitro transfection efficiencies in the presence serum. In vivo results also showed that the HGC nanoparticles had superior transfection efficiencies to naked DNA and a commercialized transfection agent. The HGC nanoparticles composed of hydrophobized DNA and hydrophobically modified glycol chitosan played a significant role in enhancing transfection efficiencies in vitro as well as in vivo. (c) 2004 Elsevier B.V. All rights reserved. English ELSEVIER SCIENCE BV BEARING 5-BETA-CHOLANIC ACID TRANSFECTION EFFICIENCY DNA NANOPARTICLES COMPLEX SYSTEM Self-assembled nanoparticles containing hydrophobically modified glycol chitosan for gene delivery Article 10.1016/j.jconrel.2004.11.033 1 JOURNAL OF CONTROLLED RELEASE, v.103, no.1, pp.235 - 243 JOURNAL OF CONTROLLED RELEASE 103 1 235 243 scie scopus 000227546800021 2-s2.0-13844265668 Chemistry, Multidisciplinary Pharmacology & Pharmacy Chemistry Pharmacology & Pharmacy Article BEARING 5-BETA-CHOLANIC ACID TRANSFECTION EFFICIENCY DNA NANOPARTICLES COMPLEX SYSTEM gene delivery chitosan self-assembled in vivo transfection