Ihm, JE Han, KO Hwang, CS Kang, JH Ahn, KD Han, IK Han, DK Hubbell, JA Cho, CS 2024-01-21T05:31:58Z 2024-01-21T05:31:58Z 2021-09-03 2005-03 1742-7061 https://pubs.kist.re.kr/handle/201004/136710 We explored poly(4-vinylimidazole) (P4V) as a nonviral gene carrier. We show that P4V can form DNA condensates of small size (< 110 nm) using a dye-exclusion assay with ethidium bromide and dynamic light scattering, and that the complexes form in a pH-sensitive manner, due to the amphotericity of the polymer. P4V was demonstrated to lead to transfection in vitro as effectively as polyethyleneimine (PEI), but at lower cytotoxicity, under conditions where higher amounts of either polymer are required, using luciferase and green fluorescent protein as examples. Transfection in vivo was also explored, using a gene encoding yellow fluorescent protein and human osteoprotegerin injected in the tail vein of the rat. Transfection was observed, both at the gene and protein levels in lung and spleen tissue. Transfection in vivo appeared to be at least as effective using P4V as with PEI. Based upon this good transfection and low cytotoxicity, P4V seems to show promise as a nonviral gene transfer vector. (c) 2004 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved. English ELSEVIER SCI LTD HISTIDYLATED POLYLYSINE DELIVERY DNA EFFICIENCY NANOPARTICLES CATALYSIS VECTORS CELLS Poly (4-vinylimidazole) as nonviral gene carrier: in vitro and in vivo transfection Article 10.1016/j.actbio.2004.12.002 1 ACTA BIOMATERIALIA, v.1, no.2, pp.165 - 172 ACTA BIOMATERIALIA 1 2 165 172 scie scopus 000235024400003 2-s2.0-33644804806 Engineering, Biomedical Materials Science, Biomaterials Engineering Materials Science Article HISTIDYLATED POLYLYSINE DELIVERY DNA EFFICIENCY NANOPARTICLES CATALYSIS VECTORS CELLS nonviral vectors gene transfer polyimidazoles