Lee, H.J. Kim, J.S. Jin, C. Ryu, J.-H. 2024-01-21T05:32:36Z 2024-01-21T05:32:36Z 2021-09-02 2005-03 1226-3907 https://pubs.kist.re.kr/handle/201004/136722 Nitric Oxide (NO), derived from L-arginine, is produced by two types (constitutive and inducible) of nitric oxide synthase (NOS: cNOS and iNOS). The NO produced in large amounts by the iNOS is known to be responsible for the vasodilation and hypotension observed in septic shock, cancer metastasis and inflammation. The inhibitors of iNOS, thus, may be useful candidates for the treatment of inflammatory diseases accompanied by the overproduction of NO. We prepared alcoholic extracts of herbal drugs which have been used for the treatment of inflammation in oriental medicine. We have screened the inhibitory activity of NO production in lipopolysaccharide (LPS)-activated macrophages after the treatment of these extracts. Among 82 kinds of extracts of herbal drugs, 35 extracts showed the potent inhibitory activity of NO production above 50% at the concentration of 50 μg/mL. The inhibitory activities of NO production were also evaluated for several solvent fractions at two different concentrations. Especially, hexane and EtOAc fractions of Alpinia officinarum, Angelica gigas, Ostericum koreanum, Saussurea lappa, Torilis japonica, and hexane fractions of Agrimonia pilosa, Machilus thunbergii, Hydrangea serrata, Magnolia obovata, Prunella vulgaris, Tussilago farfara, and EtOAC fractions of Perilla frutescence showed a significant activity at 10 and/or 25 μg/mL. In Western blot analysis, the hexane fractions (5 μg/mL) of Magnolia obovata and Saussurea lappa, and EtOAc fractions (20 μg/mL) of Hydrangea Serrata, Perilla frutescence and Torilis japonica inhibited the expression of iNOS protein in LPS-activated macrophages. These plants may be promising candidates for the study of the activity-guided purification of active compounds and might be useful for the treatment of inflammatory diseases and endotoxemia accompanying overproduction of NO. English Aconitum koreanum extract Agrimonia pilosa extract alpinia officinarum extract amomum xanthioides extract Angelica dahurica extract Angelica gigas extract Anthriscus sylvestris extract Aralia continentalis extract Areca catechu extract Aristolochia contorta extract Artemisia asiatica extract Asparagus cochinchinensis extract Aster tataricus extract Atractylodes japonica extract Bupleurum falcatum extract Carthamus tinctoris extract Hydrangea serrata extract lipopolysaccharide Machilus thunbergii extract magnolia obovata extract nitric oxide nitric oxide synthase Ostericum koreanum extract Perilla frutescence extract plant extract Prunella vulgaris extract Saussurea lappa extract Torilis japonica extract tussilago farfara extract unclassified drug unindexed drug agrimonia pilosa alpinia officinarum Angelica gigas animal cell article controlled study drug activity drug purification drug screening endotoxemia herb herbal medicine Hydrangea serrata hypotension inflammation inflammatory disease Machilus thunbergii macrophage magnolia obovata metastasis nonhuman Ostericum koreanum Perilla frutescence protein expression prunella vulgaris saussurea lappa septic shock Torilis japonica treatment indication tussilago farfara vasodilatation Western blotting Inhibitory activity of medicinal herbs on nitric oxide synthesis in activated macrophages Article 1 Natural Product Sciences, v.11, no.1, pp.16 - 21 Natural Product Sciences 11 1 16 21 scopus kci other 2-s2.0-16344384766 Article Aconitum koreanum extract Agrimonia pilosa extract alpinia officinarum extract amomum xanthioides extract Angelica dahurica extract Angelica gigas extract Anthriscus sylvestris extract Aralia continentalis extract Areca catechu extract Aristolochia contorta extract Artemisia asiatica extract Asparagus cochinchinensis extract Aster tataricus extract Atractylodes japonica extract Bupleurum falcatum extract Carthamus tinctoris extract Hydrangea serrata extract lipopolysaccharide Machilus thunbergii extract magnolia obovata extract nitric oxide nitric oxide synthase Ostericum koreanum extract Perilla frutescence extract plant extract Prunella vulgaris extract Saussurea lappa extract Torilis japonica extract tussilago farfara extract unclassified drug unindexed drug agrimonia pilosa alpinia officinarum Angelica gigas animal cell article controlled study drug activity drug purification drug screening endotoxemia herb herbal medicine Hydrangea serrata hypotension inflammation inflammatory disease Machilus thunbergii macrophage magnolia obovata metastasis nonhuman Ostericum koreanum Perilla frutescence protein expression prunella vulgaris saussurea lappa septic shock Torilis japonica treatment indication tussilago farfara vasodilatation Western blotting Herbal drug Inhibitor Macrophage Nitric oxide Nitric oxide synthase Screening