Lee, SK Park, MW Yang, EG Yu, JH Jeong, SJ 2024-01-21T05:33:40Z 2024-01-21T05:33:40Z 2021-09-03 2005-02-04 0006-291X https://pubs.kist.re.kr/handle/201004/136739 The architectural transcription factor TCF-1 interacts directly with beta-catenin and activates transcription of various target genes that are important for early development and carcinogenesis. We selected an RNA aptamer that specifically bound to the beta-catenin-interacting N-terminal motif of TCF-1. Structural analysis revealed that it formed a stem-loop structure that was responsible for binding TCF-1 and contained a pair of internal loops. The RNA aptamer interfered with the binding of TCF-1 to beta-catenin and also inhibited the formation of TCF-1/beta-catenin complexes. Disruption of TCF-1/beta-catenin complexes could alter the transcriptional activity of TCF-1. Taken together our observations show that a rationally designed RNA aptamer can disrupt protein-protein interactions required for the formation of an active transcription complex. (C) 2004 Published by Elsevier Inc. English ACADEMIC PRESS INC ELSEVIER SCIENCE TRANSCRIPTION FACTOR LEF-1 NUCLEOCAPSID PROTEIN COLON-CARCINOMA ACTIVATION DROSOPHILA ARMADILLO SELECTION LIGANDS COMPLEX APC An RNA aptamer that binds to the beta-catenin interaction domain of TCF-1 protein Article 10.1016/j.bbrc.2004.12.011 1 BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, v.327, no.1, pp.294 - 299 BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 327 1 294 299 scie scopus 000226345400042 2-s2.0-11144223772 Biochemistry & Molecular Biology Biophysics Biochemistry & Molecular Biology Biophysics Article TRANSCRIPTION FACTOR LEF-1 NUCLEOCAPSID PROTEIN COLON-CARCINOMA ACTIVATION DROSOPHILA ARMADILLO SELECTION LIGANDS COMPLEX APC TCF-1 beta-catenin RNA aptamer protein protein interaction