Yun, SH Sang, Byoung In Park, DH 2024-01-21T05:37:06Z 2024-01-21T05:37:06Z 2021-09-03 2005-02 1017-7825 https://pubs.kist.re.kr/handle/201004/136804 In this research, we examined the effect of NaCl on the growth, energy metabolism, and proton motive force of Halomonas salina, and the effect of compatible solutes on the bacterium growing in the high salinity environment. H. salina was isolated from seawater and identified by 16s-rDNA sequencing. The growth of H. salina was not enhanced by the addition of external compatible solutes (choline and betaine) in the high salinity environment. The resting cells of H. salina absorbed more glucose in the presence of 2.0 M NaCl than in its absence. H. salina did not grow in the medium with either KCl, RbCl, CsCl, Na2SO4, or NaNO3, in place of NaCl. The optimal concentration of NaCl for the growth of H. salina ranged from 1.4 M to 2.5 M, and the growth yield was decreased in the presence of NaCl below 1.4 M and above 2.5 M. The activity of isocitrate dehydrogenase, pyruvate dehydrogenase, and malate dehydrogenase of H. salina was not inhibited by NaCl in in vitro test. The proton translocation of H. salina was detected in the presence of NaCl only. These results indicate that NaCl is absolutely required for the normal growth and energy metabolism of H. salina, but the bacterial growth is not enhanced by the compatible solutes added to the growth medium. English KOREAN SOC MICROBIOLOGY & BIOTECHNOLOGY HALOTOLERANT BACTERIUM COMPATIBLE SOLUTES NEUTRAL RED ELONGATA HYDROXYECTOINE TRANSPORTER CHOLINE ECTOINE BETAINE BA-1 Influence of NaCl on the growth and metabolism of Halomonas salina Article 1 JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, v.15, no.1, pp.118 - 124 JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY 15 1 118 124 scie scopus kci 000227309000019 2-s2.0-15044365705 Biotechnology & Applied Microbiology Microbiology Biotechnology & Applied Microbiology Microbiology Article HALOTOLERANT BACTERIUM COMPATIBLE SOLUTES NEUTRAL RED ELONGATA HYDROXYECTOINE TRANSPORTER CHOLINE ECTOINE BETAINE BA-1 Halonionas salina compatible solute proton translocation salinity environment cyclic voltammetry halophilic bacterium