Hashimotodani, Y Ohno-Shosaku, T Tsubokawa, H Ogata, H Emoto, K Maejima, T Araishi, K Shin, HS Kano, M 2024-01-21T05:37:58Z 2024-01-21T05:37:58Z 2021-09-03 2005-01-20 0896-6273 https://pubs.kist.re.kr/handle/201004/136819 Endocannabinoids mediate retrograde signal and modulate transmission efficacy at various central synapses. Although endocannabinoid release is induced by either depolarization or activation of G(q/11)-coupled receptors, it is markedly enhanced by the coincidence of depolarization and receptor activation. Here we report that this coincidence is detected by phospholipase Cbeta1 (PLCbeta1) in hippocampal neurons. By measuring cannabinoid-sensitive synaptic currents, we found that the receptor-driven endocannabinoid release was dependent on physiological levels of intracellular Call concentration ([Ca2+]i), and markedly enhanced by depolarization-induced [Call]i elevation. Furthermore, we measured PLC activity in intact neurons by using exogenous TRPC6 channel as a biosensor for the PLC product diacylglycerol and found that the receptor-driven PLC activation exhibited similar [Calli dependence to that of endocannabinoid release. Neither endocannabinoid release nor PLC activation was induced by receptor activation in PLCbeta1 knockout mice. We therefore conclude that PLCbeta1 serves as a coincidence detector through its Call dependency for endocannabinoid release in hippocampal neurons. English CELL PRESS DEPOLARIZED POSTSYNAPTIC NEURONS LIVER PLASMA-MEMBRANES METABOTROPIC GLUTAMATE ENDOGENOUS CANNABINOIDS INOSITOL 1,4,5-TRISPHOSPHATE RECEPTOR REGULATION CALCIUM ACTIVATION RELEASE CELLS Phospholipase C beta serves as a coincidence detector through its Ca2+ dependency for triggering retrograde endocannabinoid signal Article 10.1016/j.neuron.2005.01.004 1 NEURON, v.45, no.2, pp.257 - 268 NEURON 45 2 257 268 scie scopus 000226451500012 2-s2.0-12344311450 Neurosciences Neurosciences & Neurology Article DEPOLARIZED POSTSYNAPTIC NEURONS LIVER PLASMA-MEMBRANES METABOTROPIC GLUTAMATE ENDOGENOUS CANNABINOIDS INOSITOL 1,4,5-TRISPHOSPHATE RECEPTOR REGULATION CALCIUM ACTIVATION RELEASE CELLS