Ha, HY Cho, IH Lee, KW Lee, KW Song, JY Kim, KS Yu, YM Lee, JK Song, JS Yang, SD Shin, HS Han, PL 2024-01-21T05:38:57Z 2024-01-21T05:38:57Z 2021-09-05 2005-01-01 0012-1606 https://pubs.kist.re.kr/handle/201004/136837 The JNK interacting protein, JSAP1, has been identified as a scaffold protein for mitogen-activated protein kinase (MAPK) signaling pathways and as a linker protein for the cargo transport along the axons. To investigate the physiological function of JSAP1 in vivo, we generated mice lacking JSAP1. The JSAP1 null mutation produced various developmental deficits in the brain, including an axon guidance defect of the corpus callosum, in which phospho-FAK and phospho-JNK were distributed at reduced levels. The axon guidance defect of the corpus callosum in the jsap1(-/-) brain was correlated with the misplacement of glial sling cells, which reverted to their normal position after the transgenic expression of JNK interacting protein 1(JIP1). The transgenic JIP1 partially rescued the axon guidance defect of the corpus callosum and the anterior commissure of the jsap1(-/-) brain. The JSAP1 null mutation impaired the normal distribution of the Ca+2 regulating protein, calretinin, but not the synaptic vesicle marker, SNAP-25, along the axons of the thalamocortical tract. These results suggest that JSAP1 is required for the axon guidance of the telencephalic commissures and the distribution of cellular protein(s) along axons in vivo, and that the signaling network organized commonly by JIP1 and JSAP1 regulates the axon guidance in the developing brain. (C) 2004 Elsevier Inc. All rights reserved. English ACADEMIC PRESS INC ELSEVIER SCIENCE FOCAL ADHESION KINASE N-TERMINAL KINASE AMYLOID PRECURSOR PROTEIN SCAFFOLD PROTEIN INTERACTING PROTEIN-1 SIGNALING PATHWAYS JNK MICE PHOSPHORYLATION JSAP1 The axon guidance defect of the telencephalic commissures of the JSAP1-deficient brain was partially rescued by the transgenic expression of JIP1 Article 10.1016/j.ydbio.2004.09.019 1 DEVELOPMENTAL BIOLOGY, v.277, no.1, pp.184 - 199 DEVELOPMENTAL BIOLOGY 277 1 184 199 scie scopus 000225741200015 2-s2.0-9644296032 Developmental Biology Developmental Biology Article FOCAL ADHESION KINASE N-TERMINAL KINASE AMYLOID PRECURSOR PROTEIN SCAFFOLD PROTEIN INTERACTING PROTEIN-1 SIGNALING PATHWAYS JNK MICE PHOSPHORYLATION JSAP1 JIP1 JSAP1 scaffold protein JNK corpus callosum anterior commissures