Lee, JH Jang, JM Cho, HS Han, KC Kim, TS Kang, JY Yang, EG 2024-01-21T05:40:15Z 2024-01-21T05:40:15Z 2021-09-05 2005-01 1013-9826 https://pubs.kist.re.kr/handle/201004/136860 Microfluidic devices are of considerable interest, since such technology offers great promise for the development of powerful and versatile miniaturized analyzers. Accordingly, the present work describes a microfluidic screening system that is composed of a microchip, hydrodynamic pumping unit and fluorescence detectors. To develop an assay for RNA-aminoglycoside interactions, microchips are designed and fabricated on a glass substrate, then flow simulations are performed in the microchannels. After optimizing the flow control and buffer composition for fluorescence-based biochemical assays, a fluorescently labeled aminoglycoside probe and RNA are allowed to flow continuously to the main micro-channel based on hydrodynamic pumping and their interactions monitored by fluorescence quenching, which is reversed upon competition with other aminoglycosides. Consequently, the proposed device can serve as an integrated microfluidic platform for the high-throughput screening of high affinity antibiotics for RNA targets. English TRANS TECH PUBLICATIONS LTD Design and characterization of microfluidic analysis system for RNA-aminoglycoside interactions Article 10.4028/www.scientific.net/KEM.277-279.90 1 ON THE CONVERGENCE OF BIO-INFORMATION-, ENVIRONMENTAL-, ENERGY-, SPACE- AND NANO-TECHNOLOGIES, PTS 1 AND 2, v.277-279, pp.90 - 95 ON THE CONVERGENCE OF BIO-INFORMATION-, ENVIRONMENTAL-, ENERGY-, SPACE- AND NANO-TECHNOLOGIES, PTS 1 AND 2 277-279 90 95 N scie scopus 000227164500017 2-s2.0-33646401122 Engineering, Multidisciplinary Materials Science, Ceramics Materials Science, Composites Engineering Materials Science Article; Proceedings Paper ELECTROPHORESIS BINDING microfluidic devices microchips hydrodynamic flow fluorescence intensity screening RNA aminoglycosides