Park, K Kim, K Kwon, IC Kim, SK Lee, S Lee, DY Byun, Y 2024-01-21T05:43:00Z 2024-01-21T05:43:00Z 2021-09-05 2004-12-21 0743-7463 https://pubs.kist.re.kr/handle/201004/136910 Various deoxycholic acid (DOCA) bearing heparin (HD) amphiphilic conjugates with different degrees of substitution (DS) with DOCA were synthesized using heparin as a hydrophilic segment and DOCA as a hydrophobic segment. Structural characteristics of these HD conjugates were investigated using H-1 NMR, dynamic light scattering, zeta potential, transmission electron microscopy (TEM), and fluorescence spectroscopy. HD conjugates provided monodispersed self-aggregates in water, with mean diameters decreasing with increasing DOCA DS in the range of 120-200 nm. HD aggregates were covered with charged heparin shells, exhibiting xi potentials near -56 mV. The critical aggregation concentration (cac) of the HD (0.02-0.003 mg/mL) depended upon DOCA DS. TEM images demonstrated that the shape of the self-aggregates was spherical. Partition equilibrium constants, K-v, for pyrene in the self-aggregates in water indicated that increasing DS enhanced the hydrophobicity of the self-aggregate inner core. The mean aggregation number of DOCA per hydrophobic microdomain, estimated by the fluorescence quenching methods using cetylpyridinium chloride, indicated that five to nine of HD chains comprised a hydrophobic domain in the conjugates. English AMER CHEMICAL SOC TERMINALLY ALKYLATED HEPARIN MODIFIED CHITOSAN BLOCK-COPOLYMER PHYSICOCHEMICAL CHARACTERISTICS HYDROGEL NANOPARTICLE SODIUM DEOXYCHOLATE AGGREGATION NUMBER WATER FLUORESCENCE MICELLES Preparation and characterization of self-assembled nanoparticles of heparin-deoxycholic acid conjugates Article 10.1021/la048646i 1 LANGMUIR, v.20, no.26, pp.11726 - 11731 LANGMUIR 20 26 11726 11731 scie scopus 000225816800061 2-s2.0-11144247937 Chemistry, Multidisciplinary Chemistry, Physical Materials Science, Multidisciplinary Chemistry Materials Science Article TERMINALLY ALKYLATED HEPARIN MODIFIED CHITOSAN BLOCK-COPOLYMER PHYSICOCHEMICAL CHARACTERISTICS HYDROGEL NANOPARTICLE SODIUM DEOXYCHOLATE AGGREGATION NUMBER WATER FLUORESCENCE MICELLES nanoparticle deoxycholic acid EPR cancer