Kim, MS Choie, WS Shin, YS Yu, MH Lee, SW 2024-01-21T05:43:03Z 2024-01-21T05:43:03Z 2021-09-05 2004-12-20 0253-2964 https://pubs.kist.re.kr/handle/201004/136911 Recently mass spectrometry and separation methods such as liquid chromatography have become major tools in the field of proteomics. In this report, we describe in detail our efforts to develop ultra-high pressure capillary reverse-phase liquid chromatography (cRPLC) and its online coupling to a mass spectrometer by a nanoelectrospray (nanoESI) interface. The RPLC system is constructed in house to deliver LC solvents at the pressure up to 20,000 psig, which is four times higher than conventional RPLC systems. The high operation pressure allows the efficient use of packed micro-capillary columns (50, 75 and 150,mum i.d., up to 1.5 in long). We will discuss the effect of column diameter on the sensitivity of cRPLC/MS/MS experiments and the utility of the developed technique for proteome analysis by its application in the analysis of proteome samples having different levels of complexity. English WILEY-V C H VERLAG GMBH PROTEIN IDENTIFICATION TECHNOLOGY YEAST PROTEOME SCALE ONLINE Development of ultra-high pressure capillary reverse-phase liquid chromatography/tandem mass spectrometry for high-sensitive and high-throughput proteomics Article 1 BULLETIN OF THE KOREAN CHEMICAL SOCIETY, v.25, no.12, pp.1833 - 1839 BULLETIN OF THE KOREAN CHEMICAL SOCIETY 25 12 1833 1839 scie scopus kci ART000942291 000226854800015 2-s2.0-13544254427 Chemistry, Multidisciplinary Chemistry Article PROTEIN IDENTIFICATION TECHNOLOGY YEAST PROTEOME SCALE ONLINE proteomics liquid chromatography tandem mass spectrometry