Yune, TY Lee, SM Kim, SJ Park, HK Oh, YJ Kim, YC Markelonis, GJ Oh, TH 2024-01-21T06:03:39Z 2024-01-21T06:03:39Z 2021-09-04 2004-12 0897-7151 https://pubs.kist.re.kr/handle/201004/137008 Antioxidant enzymes including superoxide dismutase (SOD) may play a role in the mechanism by which cells counteract the deleterious effects of reactive oxygen species (ROS) after spinal cord injury (SCI). Cu/Zn and MnSOD are especially potent scavengers of superoxide anion and likely serve important cytoprotective roles against cellular damage. We investigated expression of SOD after SCI to address its role during the early stages of injury. MnSOD activity was increased 4 h after SCI and persisted at elevated levels up to 24-48 h; by contrast, Cu/ZnSOD activity was not changed. `RT-PCR and Western blot analyses showed increased levels of MnSOD mRNA and protein, respectively, by 4 h and reached maximum levels by 24-48 h. Double immunostaining revealed that MnSOD protein was localized within neurons and oligodendrocytes. Tumor necrosis factor-alpha (TNFalpha) was administered locally into uninjured spinal cords to examine potential mechanisms for MnSOD induction after injury. TNF-alpha administered exogenously increased MnSOD expression in uninjured spinal cords. Western blot and immunostaining also revealed that a transcription factor, NF-kappaB, was activated and translocated into the nuclei of neurons and oligodendrocytes. By contrast, administration of neutralizing antibody against TNF-alpha into injured spinal cords attenuated the increase in MnSOD expression and activation of NF-kappaB. Double immunostaining revealed that MnSOD was co-localized with NF-kappaB in neurons and oligodendrocytes after SCI. These results suggest that TNF-alpha may be an inducer of NF-kappaB activation and MnSOD expression after SCI and that MnSOD expression induced by TNF-alpha is likely mediated through activation of NF-kappaB. English MARY ANN LIEBERT, INC TUMOR-NECROSIS-FACTOR FOCAL CEREBRAL-ISCHEMIA TRAUMATIC BRAIN-INJURY MITOCHONDRIAL CYTOCHROME-C ENDOMETRIAL STROMAL CELLS OXIDATIVE STRESS TRANSGENIC MICE MESSENGER-RNA NEURODEGENERATIVE DISEASES FUNCTIONAL RECOVERY Manganese superoxide dismutase induced by TNF-alpha is regulated transcriptionally by NF-kappa B after spinal cord injury in rats Article 10.1089/neu.2004.21.1778 1 JOURNAL OF NEUROTRAUMA, v.21, no.12, pp.1778 - 1794 JOURNAL OF NEUROTRAUMA 21 12 1778 1794 scie scopus 000225554400009 2-s2.0-9744246881 Critical Care Medicine Clinical Neurology Neurosciences General & Internal Medicine Neurosciences & Neurology Article TUMOR-NECROSIS-FACTOR FOCAL CEREBRAL-ISCHEMIA TRAUMATIC BRAIN-INJURY MITOCHONDRIAL CYTOCHROME-C ENDOMETRIAL STROMAL CELLS OXIDATIVE STRESS TRANSGENIC MICE MESSENGER-RNA NEURODEGENERATIVE DISEASES FUNCTIONAL RECOVERY Cu/ZnSOD MnSOD NF-kappa B spinal cord injury TNF-alpha TNF-alpha neutralizing antibody