Kim, Y Min, HY Park, HJ Lee, EJ Park, EJ Hwang, HJ Jin, C Lee, YS Lee, SK 2024-01-21T06:14:54Z 2024-01-21T06:14:54Z 2021-09-01 2004-10 0951-418X https://pubs.kist.re.kr/handle/201004/137215 Nitric oxide (NO) produced by inducible nitric oxide synthase (iNOS) has played a crucial role in various pathophysiological processes including inflammation and carcinogenesis. Therefore, the inhibitors of NO synthesis or iNOS gene expression have been considered as potential anti-inflammatory and cancer chemopreventive agents. In our continuous search for iNOS inhibitors from natural products we have evaluated indigenous Korean plant extracts using an assay for inhibition of nitric oxide formation on lipopolysaccharide (LPS)activated mouse macrophage RAW 264.7 cells. As a result, the methanolic stem extract of Actinodaphne lancifolia showed an inhibitory activity of NO production in a dose-dependent manner (IC50 = 2.5 mug/ml). Additional study demonstrated that the extract of Actinodaphne lancifolia significantly suppressed the iNOS protein and gene expression in a dose-dependent manner. These results suggest that Actinodaphne lancifolia could be a potential candidate for developing an iNOS inhibitor from natural products. Further elucidation of active principles for development of new cancer chemopreventive and/or anti-inflammatory agents could be warranted. Copyright (C) 2004 John Wiley Sons, Ltd. English WILEY CANCER CARCINOGENESIS MACROPHAGES PROGRESSION INHIBITION GENE HEAD NECK Suppressive effect of inducible nitric oxide synthase (iNOS) expression by the methanol extract of Actinodaphne lancifolia Article 10.1002/ptr.1558 1 PHYTOTHERAPY RESEARCH, v.18, no.10, pp.853 - 856 PHYTOTHERAPY RESEARCH 18 10 853 856 scie scopus 000225455900015 2-s2.0-9644304538 Chemistry, Medicinal Pharmacology & Pharmacy Pharmacology & Pharmacy Article CANCER CARCINOGENESIS MACROPHAGES PROGRESSION INHIBITION GENE HEAD NECK inducible nitric oxide synthase (NOS) Actinodaphne lancifolia lipopolysaccharide (LPS) nitric oxides RAW 264.7 cells