Son, J Lee, J Lee, M Lee, E Lee, KT La, S Kim, DH 2024-01-21T06:15:27Z 2024-01-21T06:15:27Z 2021-09-01 2004-09-21 0731-7085 https://pubs.kist.re.kr/handle/201004/137223 A rapid and accurate reversed-phase liquid chromatography-tandem mass spectrometry (LC-MS/MS) method has been developed and validated for the quantitative determination of oxiracetam in human plasma. After addition of internal standard (piracetam) plasma was precipitated with two volumes of acetonitrile and the supernatant was evaporated. The residues were dissolved in 0.1% acetic acid and analyzed by reversed-phase HPLC with the detection of the analyte in the multiple reaction monitoring (MRM) mode. This method for the determination of oxiracetam was accurate and reproducible, with a limit of quantitation of 0.2 mug/ml in human plasma. The standard calibration curve for oxiracetam was linear (r(2) = 0.999) over the concentration range 0.2-40.0 mug/ml in human plasma. The intra- and inter-day precision over the concentration range of oxiracetam was lower than 8.3% (relative standard deviation, %R.S.D.), and accuracy was between 92.5-106.4%. (C) 2004 Elsevier B.V. All rights reserved. English ELSEVIER SCIENCE BV URINE Rapid quantitative analysis of oxiracetam in human plasma by liquid chromatography/electrospray tandem mass spectrometry Article 10.1016/j.jpba.2004.05.010 1 JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS, v.36, no.1, pp.183 - 187 JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS 36 1 183 187 scie scopus 000224328000024 2-s2.0-7444248417 Chemistry, Analytical Pharmacology & Pharmacy Chemistry Pharmacology & Pharmacy Article URINE oxiracetam pharmacokinetics LC-MS/MS multiple reaction monitoring