Back, JH Kim, MS Cho, H Chang, IS Lee, JY Kim, KS Kim, BH Park, YI Han, YS 2024-01-21T06:31:43Z 2024-01-21T06:31:43Z 2021-09-05 2004-09-01 0378-1097 https://pubs.kist.re.kr/handle/201004/137241 A microbial fuel cell is a device that directly converts metabolic energy into electricity, using electrochemical technology. The analysis of large genome fragments recovered directly from microbial communities represents one promising approach to characterizing uncultivated electrochemical microorganisms. To further assess the utility of this approach, we constructed large-insert (140 kb) bacterial artificial chromosome (BAC) libraries from the genomic DNA of a microbial fuel cell, which had been operated for three weeks using acetate media. We screened the expression of several ferric reductase activities in the Escherichia coli host, in order to determine the extent of heterologous expression of metal-ion-reducing enzymes in the library. Phylogenetic analysis of 16S rRNA gene sequences recovered from the BAC libraries indicates that they contain DNA from a wide diversity of microbial organisms. The constructed bacterial library proved a powerful tool for exploring metal-ion reductase activities, providing information on the electron transport pathway of electrochemical microbial (ECM) organisms. (C) 2004 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved. English OXFORD UNIV PRESS SHEWANELLA-PUTREFACIENS MR-1 MICROBIAL FUEL-CELL ESCHERICHIA-COLI IRON CYTOCHROME REDUCTION ENVIRONMENTS EXPRESSION MANGANESE Construction of bacterial artificial chromosome library from electrochemical microorganisms Article 10.1016/j.femsle.2004.07.018 1 FEMS MICROBIOLOGY LETTERS, v.238, no.1, pp.65 - 70 FEMS MICROBIOLOGY LETTERS 238 1 65 70 scie scopus 000223805600010 2-s2.0-4444328060 Microbiology Microbiology Article SHEWANELLA-PUTREFACIENS MR-1 MICROBIAL FUEL-CELL ESCHERICHIA-COLI IRON CYTOCHROME REDUCTION ENVIRONMENTS EXPRESSION MANGANESE electrochemical microbial organisms bacterial artificial chromosome library microbial fuel cell metal reductase