Babu, CVS Lee, J Lho, DS Yoo, YS 2024-01-21T06:35:53Z 2024-01-21T06:35:53Z 2021-09-05 2004-08-05 1570-0232 https://pubs.kist.re.kr/handle/201004/137317 Interest in the analysis of low abundance neuropeptides particularly using matrix-assisted laser desorption/ionization time-of-flight mass-spectrometry (MALDI-TOF-MS) is increasing because these neuropeptides are essential to the mechanism of transportation and the metabolism. This article describes an immunoprecipitation procedure that is suitable for MALDI-MS analysis of substance P (SP), a neuropeptide, in rat brain tissues. Substance P was precipitated from brain tissue extracts by immunoprecipitation with antibodies directed against SP, and are analyzed by MALDI-TOF-MS. Mass spectrometric analysis showed a singly charged [M + H](+) ion peak that corresponded to the SP molecular mass and was observed with a detection error of 1.6%. The average mass errors between the observed and theoretical molecular mass were within the 0.11 Da range. Capillary zone electrophoresis analysis was subsequently performed, and the effects of the different separation parameters were examined. Beginning with milligram quantities of brain tissue, picomole quantities of SP could be detected using this method. (C) 2004 Elsevier B.V. All rights reserved. English ELSEVIER SCIENCE BV DESORPTION IONIZATION CEREBROSPINAL-FLUID NEUROPEPTIDE-Y ELECTROPHORESIS IDENTIFICATION SOMATOSTATIN PEPTIDES Analysis of substance P in rat brain by means of immunoaffinity capture and matrix-assisted laser desorption/ionization time-of-flight mass-spectrometry Article 10.1016/j.jchromb.2004.04.026 1 JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, v.807, no.2, pp.307 - 313 JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 807 2 307 313 scie scopus 000222283800020 2-s2.0-2942622478 Biochemical Research Methods Chemistry, Analytical Biochemistry & Molecular Biology Chemistry Article DESORPTION IONIZATION CEREBROSPINAL-FLUID NEUROPEPTIDE-Y ELECTROPHORESIS IDENTIFICATION SOMATOSTATIN PEPTIDES substance P