Lee, SH Jung, BH Kim, SY Chung, BC 2024-01-21T06:37:59Z 2024-01-21T06:37:59Z 2021-09-05 2004-08 0955-2863 https://pubs.kist.re.kr/handle/201004/137356 Quantitative analytical methods for the 17 phytoestrogens containing isoflavonoids, lignans, and mycoestrogens in herbs were developed, and the amount of phytoestrogens was determined in 22 traditional medicinal herbs. The focus of this study was to simplify the purification procedure for removing many kinds of interferences in herbs, and to select adequate derivatization reagent for getting desirable selectivity and sensitivity in the quantitative determination of phytoestrogens. To satisfy these goals, we performed a solid-phase extraction with Oasis HLB cartridges following enzymatic and acidic hydrolysis, and we used the mixture of MSTFA/NH4I/DTE (1000:4:5, v/w/w) to form TMS derivatives of phytoestrogens. Overall recovery was more than 84% in all of the phytoestrogens, and the limit of quantification for phytoestrogens in herbs were set at 0.2 mug/g. Coefficient of variation percentages were in the range of 0.18-15.68% (within-day) and 0.23-16.61% (day-to-day), respectively. Most of the isoflavonoids and lignans were found in all of the herbs, but mycoestrogens were not detected at all. The Leguminosae family proved to be the richest source of isoflavonoids. Lignans such as enterodiol and enterolactone were detected at low concentration in most of the herbs. These results indicate that this assay is accurate and reliable for the determination of phytoestrogens in herbs. Also, information regarding the phytoestrogen contents in traditional medicinal herbs is useful in the prevention and treatment of chronic diseases such as cancer, osteoporosis, dementia, and cardiovascular disease. (C) 2004 Elsevier Inc. All rights reserved. English ELSEVIER SCIENCE INC PREVENTS BONE LOSS BREAST FOOD ISOFLAVONOIDS RESVERATROL PROSTATE LIGNANS CANCER Determination of phytoestrogens in traditional medicinal herbs using gas chromatography-mass spectrometry Article 10.1016/j.jnutbio.2004.01.007 1 JOURNAL OF NUTRITIONAL BIOCHEMISTRY, v.15, no.8, pp.452 - 460 JOURNAL OF NUTRITIONAL BIOCHEMISTRY 15 8 452 460 scie scopus 000223649800002 2-s2.0-4344559602 Biochemistry & Molecular Biology Nutrition & Dietetics Biochemistry & Molecular Biology Nutrition & Dietetics Article PREVENTS BONE LOSS BREAST FOOD ISOFLAVONOIDS RESVERATROL PROSTATE LIGNANS CANCER gas chromatography-mass spectrometry (GC-MS) phytoestrogens traditional medicinal herb isoflavonoids lignans