Kwon, OS Chung, YB 2024-01-21T07:04:05Z 2024-01-21T07:04:05Z 2021-09-02 2004-06 0253-6269 https://pubs.kist.re.kr/handle/201004/137554 Acetyl-L-carnitine (ALC), a naturally occurring endogenous compound, has been shown to improve the cognitive performance of patients with senile dementia Alzheimer's type, and to be involved in cholinergic neurotransmission. Because ALC is an endogenous compound, validation of the analytical methods of ALC in the biological fluids is very important and difficult. This study was presented validation and correction for plasma ALC concentrations and pharmacokinetics after oral administration of ALC to human volunteers. ALC concentrations in human plasma were corrected by subtracting the concentration of blank plasma from each sample. Precision and accuracy (bias %) for uncorrected ALC concentrations were below 2.6 and 6.5% for intra-days, and 4.0 and 9.4% for inter-days, respectively. Precision and accuracy (bias %) for corrected ALC concentrations were below 10.9 and 6.0% for intra-days, and 10.5 and 16.9% for inter-days, respectively. Quantitation limit was 0.1 mug/mL. After oral administration of a 500 mg ALC tablet to 8 healthy volunteers, the principle pharmacokinetic parameters were 14.2 h of the half-life (t(1/2,beta)), the area under the curve (AUC(0-->8)) of 9.88 mug(.)h/mL, and 3.1 h of the time (T-max) to reach C-max. This study first describes the pharmacokinetic study after oral administration of a single dose of ALC in human volunteers. English PHARMACEUTICAL SOC KOREA HPLC determination and pharmacokinetics of endogenous acetyl-L-carnitine (ALC) in human volunteers orally administered a single dose of ALC Article 10.1007/BF02980169 1 ARCHIVES OF PHARMACAL RESEARCH, v.27, no.6, pp.676 - 681 ARCHIVES OF PHARMACAL RESEARCH 27 6 676 681 N scie scopus kci 000222363000017 2-s2.0-21644478786 Chemistry, Medicinal Pharmacology & Pharmacy Pharmacology & Pharmacy Article PERFORMANCE LIQUID-CHROMATOGRAPHY PROPIONYL-L-CARNITINE HUMAN-URINE ACYLCARNITINES DERIVATIZATION METABOLISM ACETYLCARNITINE QUANTIFICATION IMPAIRMENT MEMBRANES acetyl-L-carnitine endogenous compound high-performance liquid chromatography (HPLC) validation pharamcokinetics