Lee, SH Jung, BH Kim, SY Chung, BC 2024-01-21T07:07:35Z 2024-01-21T07:07:35Z 2021-09-02 2004-05 0951-4198 https://pubs.kist.re.kr/handle/201004/137619 Oxidized nucleosides are biochemical markers for tumors, aging, and neurodegenerative diseases. However, during the last decade, the analytical methods for nucleosides by gas chromatography/ mass spectrometry (GC/MS) and high-performance liquid chromatography (HPLC) with single-parameter detectors like electron-capture detection (ECD) have not been sufficiently rapid or reliable to detect nucleosides in urine and to analyze clinical samples. It has been reported (Dudley et al., Rapid Commun Mass Spectrom. 2000; 14: 1200) that liquid chromatography with electrospray mass spectrometry (LC/ESI-MS) is more specific and sensitive for analysis of nucleosides than HPLC with conventional detectors; however, this method required complex extraction steps. In the present work a direct LC/ESI-MS method for nucleosides without extraction of urine samples has been developed. Analysis of nucleosides using positive-ion mode with selected reaction monitoring effectively eliminated potential interferences from endogenous constituents of the urine. This highly selective and sensitive method made it possible to analyze urinary nucleosides with a lower limit of quantitation of 0.2 nmol/mL. The method has been validated, with both excellent linearity and reproducibility, in the calibration range from 0.2-400 nmol/mL. The correlation coefficients of the calibration curves were higher than 0.987. The coefficients of variation were in the range 0.03-14.92% (inter-day) and 0.54-14.39% (intra-day), respectively. Copyright (C) 2004 John Wiley Sons, Ltd. English WILEY OXIDATIVE DNA-DAMAGE MASS-SPECTROMETRY CEREBROSPINAL-FLUID CANCER-PATIENTS 8-HYDROXY-2&apos -DEOXYGUANOSINE RADIATION 5-METHYL-2&apos -DEOXYCYTIDINE 8-HYDROXYGUANOSINE PSEUDOURIDINE LEUKEMIA A rapid and sensitive method for quantitation of nucleosides in human urine using liquid chromatography/mass spectrometry with direct urine injection Article 10.1002/rcm.1400 1 RAPID COMMUNICATIONS IN MASS SPECTROMETRY, v.18, no.9, pp.973 - 977 RAPID COMMUNICATIONS IN MASS SPECTROMETRY 18 9 973 977 scie scopus 000221228500008 2-s2.0-2442624501 Biochemical Research Methods Chemistry, Analytical Spectroscopy Biochemistry & Molecular Biology Chemistry Spectroscopy Article OXIDATIVE DNA-DAMAGE MASS-SPECTROMETRY CEREBROSPINAL-FLUID CANCER-PATIENTS 8-HYDROXY-2&apos -DEOXYGUANOSINE RADIATION 5-METHYL-2&apos -DEOXYCYTIDINE 8-HYDROXYGUANOSINE PSEUDOURIDINE LEUKEMIA nucleosides urine HPLC/MS direct injection