Kim, KM Jung, BH Paeng, KJ Kim, SW Chung, BC 2024-01-21T07:07:42Z 2024-01-21T07:07:42Z 2021-09-02 2004-05 0952-3278 https://pubs.kist.re.kr/handle/201004/137621 F-2-isoprostanes are derived in vivo principally from the following: (1) the formation of positional peroxyl radicals of arachidonic acid, (2) endocyclization to prostaglandin G(2)-like structures, and (3) reduction to PGF(2)-like compounds. F-2-isoprostanes have been proposed as biomarkers of lipid peroxidation, oxidative stress status, and the oxidation of low-density lipoprotein (LDL). Using gas chromatography-ion trap-mass spectrometry, we studied how hemodialysis (HD) affects plasma total F-2-isoprostanes. We examined the plasma total F-2-isoprostanes in end-stage renal disease (ESRD) patients, before HD, after HD, between HD, and in control subjects. Plasma concentrations of total F-2-isoprostanes were significantly higher in the after HD ESRD patients than the before hemodialysis ESRD patients (P<0.05). There is no difference between before HD ESRD patients and normal controls. Moreover, a positive or negative correlation was seen between LDL and plasma total F-2-isoprostanes (P<0.001), and between age and plasma total F-2-isoprostanes (P<0.001). This study indicates HD treatment may be the major contributor of oxidative stress in ESRD patients. (C) 2003 Elsevier Ltd. All rights reserved. English ELSEVIER SCI LTD LIPID-PEROXIDATION OXIDATIVE STRESS OXIDANT STRESS FREE-RADICALS IN-VIVO ISOPROSTANES FAILURE HUMANS Alteration of plasma total F-2-isoprostanes before and after hemodialysis in end-stage renal disease patients Article 10.1016/j.plefa.2003.10.001 1 PROSTAGLANDINS LEUKOTRIENES AND ESSENTIAL FATTY ACIDS, v.70, no.5, pp.475 - 478 PROSTAGLANDINS LEUKOTRIENES AND ESSENTIAL FATTY ACIDS 70 5 475 478 scie scopus 000220942000008 2-s2.0-1842684040 Biochemistry & Molecular Biology Cell Biology Endocrinology & Metabolism Biochemistry & Molecular Biology Cell Biology Endocrinology & Metabolism Article LIPID-PEROXIDATION OXIDATIVE STRESS OXIDANT STRESS FREE-RADICALS IN-VIVO ISOPROSTANES FAILURE HUMANS F-isoprostanes oxidative stress hemodialysis