Son, J Lee, J Han, CK Kwak, WJ Yang, R Kim, DH 2024-01-21T07:35:16Z 2024-01-21T07:35:16Z 2021-09-02 2004-02-09 0003-2670 https://pubs.kist.re.kr/handle/201004/137851 A column-switching high-performance liquid chromatography (HPLC) method is described for the simultaneous determination of loganin and sweroside, which are the active ingredients of purified herbal extract from Lonicera japonica (SKL JI), in rat plasma using column-switching and ultraviolet (U-V) absorbance detection. Plasma was simply filtrated prior to injection to the HPLC system consisting of a clean-up column, a concentrating column, and an analytical column, which were connected with two six-port switching valves. Detection of loganin and sweroside was accurate and repeatable, with a limit of quantitation of 0.05 mug ml(-1) in plasma. The calibration curves for both loganin and sweroside were linear over the concentration ranges of 0.05-40.0 and 0.02-40.0 mug ml(-1) in rat plasma, respectively. The intra- and inter-day precision over the concentration range for loganin and sweroside were lower than 8.1 and 10.9% (relative standard deviation, R.S.D.), and accuracy was between 94.7 and 113.5 and 95.0 and 113.1%, respectively. This method has been successfully applied to determine the levels of loganin and sweroside in rat plasma samples from pharmacokinetics studies. (C) 2003 Elsevier B.V. All rights reserved. English ELSEVIER SCIENCE BV SECOIRIDOID GLYCOSIDES DIRECT-INJECTION LIVER-INJURY MICE Simultaneous quantitation of loganin and sweroside in plasma using column-switching high-performance liquid chromatography Article 10.1016/j.aca.2003.10.036 1 ANALYTICA CHIMICA ACTA, v.503, no.2, pp.257 - 262 ANALYTICA CHIMICA ACTA 503 2 257 262 scie scopus 000188050000013 2-s2.0-0347418172 Chemistry, Analytical Chemistry Article SECOIRIDOID GLYCOSIDES DIRECT-INJECTION LIVER-INJURY MICE column-switching loganin sweroside SKL JI pharmacokinetics