Kim, BH Park, HS Kim, HJ Kim, GT Chang, IS Lee, J Phung, NT 2024-01-21T07:37:11Z 2024-01-21T07:37:11Z 2021-09-02 2004-02 0175-7598 https://pubs.kist.re.kr/handle/201004/137887 A fuel cell was used to enrich a microbial consortium generating electricity, using organic wastewater as the fuel. Within 30 days of enrichment the maximum current of 0.2 mA was generated with a resistance of 1 kOmega. Current generation was coupled to a fall in chemical oxygen demand from over 1,700 mg l(-1) down to 50 mg l(-1). Denaturing gradient gel electrophoresis showed a different microbial population in the enriched electrode from that in the sludge used as the inoculum. Electron microscopic observation showed a biofilm on the electrode surface and microbial clumps. Nanobacteria-like particles were present on the biofilm surface. Metabolic inhibitors and electron acceptors inhibited the current generation. 16S ribosomal RNA gene analysis showed a diverse bacterial population in the enrichment culture. These findings demonstrate that an electricity-generating microbial consortium can be enriched using a fuel cell and that the electrochemical activity is a form of anaerobic electron transfer. English SPRINGER-VERLAG NONCHLORINATED AROMATIC-COMPOUNDS IRON-REDUCING CONDITIONS FE(III)-REDUCING BACTERIUM SHEWANELLA-PUTREFACIENS ELECTRODE-REACTION WATER INTERFACE FE(III) OXIDE NEUTRAL RED REDUCTION FERMENTATION Enrichment of microbial community generating electricity using a fuel-cell-type electrochemical cell Article 10.1007/s00253-003-1412-6 1 APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, v.63, no.6, pp.672 - 681 APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 63 6 672 681 scie scopus 000189195300008 2-s2.0-1542329064 Biotechnology & Applied Microbiology Biotechnology & Applied Microbiology Article NONCHLORINATED AROMATIC-COMPOUNDS IRON-REDUCING CONDITIONS FE(III)-REDUCING BACTERIUM SHEWANELLA-PUTREFACIENS ELECTRODE-REACTION WATER INTERFACE FE(III) OXIDE NEUTRAL RED REDUCTION FERMENTATION microbial fuel cell microbial community