Lee, SH Yang, YJ Kim, KM Chung, BC 2024-01-21T08:02:41Z 2024-01-21T08:02:41Z 2021-09-03 2003-11-25 0304-3835 https://pubs.kist.re.kr/handle/201004/138074 The risk of cancer of the cervix is linked with sexual behavior. Although infectious agents, such as human papillomaviruses (HPVs) are implicated, these alone may be insufficient to induce the disease. We investigated the potential role of estrogen, androgen, and polyamine metabolism as co-factors in the development of cervical cancer. We obtained urine samples from patients with benign cervical disease (n = 18) and cervical cancer (n = 18) and from age-matched normal female subjects (n = 25). For 11 polyamine determination, an improved and sensitive gas-chromatographic with nitrogen/phosphorus-detection (GC/NPD) procedure was used. The urinary levels of 25 androgens and corticoids and 16 estrogens were quantitatively determined by gas chromatography-mass spectrometry-selected ion-monitoring (GC/MS/SIM). In the patients with cervical cancer, the ratio of 16alpha-hydroxy estrone (16alpha-OH E1)/2-hydroxy estrone (2-OH E1), putrescine (Put)/N-1-acetylspermidine (N-1-aeSpd) and 5beta-tetrahydrocortisol (THF)/5alpha-tetrahydrocortisol (5alpha-THF) were significantly increased in comparison to the values of the normal controls. These data suggest: (1) an increase of 16alpha-hydroxylation in estrogen metabolism; (2) the high activity of polyamine oxidase (PAO) in polyamine metabolism; and (3) the low activity of 5alpha-reductase in androgen metabolism may play a significant role in the development of cervical cancer. Although additional research is necessary, the combination of 16alpha-OH E1/2-OH E1 and THF/5alpha-THF may provide a dual marker for the discrimination of benign cervical disease and cervical cancer. (C) 2003 Elsevier Ireland Ltd. All rights reserved. English ELSEVIER IRELAND LTD HUMAN PAPILLOMAVIRUS INFECTION BREAST DISEASE ESTROGEN PROGESTERONE WOMEN METABOLISM NEOPLASIA CARCINOMA UTERINE RISK Altered urinary profiles of polyamines and endogenous steroids in patients with benign cervical disease and cervical cancer Article 10.1016/S0304-3835(03)00014-4 1 CANCER LETTERS, v.201, no.2, pp.121 - 131 CANCER LETTERS 201 2 121 131 scie scopus 000186749100001 2-s2.0-0242384110 Oncology Oncology Article HUMAN PAPILLOMAVIRUS INFECTION BREAST DISEASE ESTROGEN PROGESTERONE WOMEN METABOLISM NEOPLASIA CARCINOMA UTERINE RISK cervical cancer polyamines estrogens androgens corticoids gas chromatography-mass spectrometry gas chromatography-nitrogen-phosphorus detection