Kim, WH Chung, JH Back, JH Choi, JY Cha, JH Koh, HY Han, YS 2024-01-21T08:10:43Z 2024-01-21T08:10:43Z 2022-01-11 2003-09-30 1225-8687 https://pubs.kist.re.kr/handle/201004/138224 NAD(P)H quinone oxidoreductase is a ubiquitous enzyme that is known to directly reduce quinone substrates to hydroquinones by a two-electron reaction, We report the identification of NADPH quinone oxidoreductase from Kluyveromyces marxianus (KmQOR), which reduces quinone substrates directly to hydroquinones. The KmQOR gene was sequenced, expressed in Escherichia coli, purified, and characterized. The open-reading frame of the KmQOR gene consists of 1143 nucleotides, encoding a 380 amino acid polypeptide. The nucleotide sequence of the KmQOR gene was assigned to EMBL under accession number AY040868. The M, that was determined by SDS-PAGE for the protein subunit was about 42 kDa, and the molecular mass of the native KmQOR was 84 kDa, as determined by column calibration, indicating that the native protein is a homodimer. The KmQOR protein efficiently reduced 1,4-benzoquinone, whereas no activities were found for menadiones and methoxyquinones. These observations, and the result of an extended sequence analysis of known NADPH quinone oxidoreductase, suggest that KmQOR possesses a different action mechanism. English SPRINGER SINGAPORE PTE LTD ELECTRON TRANSFER-REACTIONS DT-DIAPHORASE NAD(P)H-QUINONE OXIDOREDUCTASE SACCHAROMYCES-CEREVISIAE HETEROLOGOUS EXPRESSION BIOCHEMICAL SYSTEMS REDUCTASE CELLS TOXICITY NAD(P)H Molecular cloning and characterization of an NADPH quinone oxidoreductase from Kluyveromyces marxianus Article 1 JOURNAL OF BIOCHEMISTRY AND MOLECULAR BIOLOGY, v.36, no.5, pp.442 - 449 JOURNAL OF BIOCHEMISTRY AND MOLECULAR BIOLOGY 36 5 442 449 scie scopus kci ART000887761 000185546700003 2-s2.0-0242319790 Biochemistry & Molecular Biology Biochemistry & Molecular Biology Article ELECTRON TRANSFER-REACTIONS DT-DIAPHORASE NAD(P)H-QUINONE OXIDOREDUCTASE SACCHAROMYCES-CEREVISIAE HETEROLOGOUS EXPRESSION BIOCHEMICAL SYSTEMS REDUCTASE CELLS TOXICITY NAD(P)H 1,4-benzoquinone Kluyveromyces marxianus NADPH quinone oxidoreductase quinones