Han, KY Ban, EM Yoo, YS 2024-01-21T08:10:46Z 2024-01-21T08:10:46Z 2021-09-03 2003-09-26 0021-9673 https://pubs.kist.re.kr/handle/201004/138225 A competitive immunoassay based on CE-LIF has been developed for the determination of vasopressin in biological mixtures. Vasopressin participates in the hormonal control of water metabolism and the constriction of arterioles in humans. Thus, detection of vasopressin is important in diagnosing pathological conditions and physiological water metabolism. The peptides were fluorescently tagged with FITC and purified by HPLC. The purified product was then mixed with the cerebrospinal fluid sample followed with the addition of anti-vasopressin antibody. It was possible to separate antibody-bound and free FITC-tagged vasopressin within 10 min by CE-LIF analysis using uncoated fused-silica capillary with high reproducibility. (C) 2003 Elsevier B.V. All rights reserved. English ELSEVIER SCIENCE BV NEUROHYPOPHYSEAL HORMONES ZONE ELECTROPHORESIS PEPTIDES CHANNELS INSULIN FISH Analysis of vasopressin using capillary electrophoresis with laser-induced fluorescence detector based on competitive immunoassay Article 10.1016/S0021-9673(03)01432-8 1 JOURNAL OF CHROMATOGRAPHY A, v.1013, no.1-2, pp.215 - 220 JOURNAL OF CHROMATOGRAPHY A 1013 1-2 215 220 scie scopus 000185656900022 2-s2.0-0142124256 Biochemical Research Methods Chemistry, Analytical Biochemistry & Molecular Biology Chemistry Article; Proceedings Paper NEUROHYPOPHYSEAL HORMONES ZONE ELECTROPHORESIS PEPTIDES CHANNELS INSULIN FISH immunoassays vasopressin peptides