Chang, IS Ballard, JD Krumholz, LR 2024-01-21T08:15:10Z 2024-01-21T08:15:10Z 2021-09-01 2003-09 0167-7012 https://pubs.kist.re.kr/handle/201004/138306 Chimeric sequences were observed to occur abundantly (48% of clones) during random arbitrarily primed polymerase chain reaction (RAP-PCR) experiments designed to examine differential expression of genes involved in metal resistance in sulfate-reducing bacteria (SRB). Some of the chimeric sequences were composed of sequence from a gene differentially expressed under the imposed conditions and a sequence of the 16S or 23S rRNA gene. The remainder were composed of two rRNA sequences. Experiments using PCR and genomic sequence analysis showed that the chimeric sequences were not due to a genetic mutation (e.g., recombination, transposition). As RAP-PCR has been widely used to identify differentially expressed genes, this observation may aid in our interpretation of RAP-PCR data. (C) 2003 Elsevier Science B.V. All rights reserved. English ELSEVIER DIFFERENTIAL DISPLAY MESSENGER-RNA FALSE POSITIVES SULFATE Evidence for chimeric sequences formed during random arbitrarily primed PCR Article 10.1016/S0167-7012(03)00093-9 1 JOURNAL OF MICROBIOLOGICAL METHODS, v.54, no.3, pp.427 - 431 JOURNAL OF MICROBIOLOGICAL METHODS 54 3 427 431 scie scopus 000184302000015 2-s2.0-0038798529 Biochemical Research Methods Microbiology Biochemistry & Molecular Biology Microbiology Article DIFFERENTIAL DISPLAY MESSENGER-RNA FALSE POSITIVES SULFATE PCR