Choi, J Chang, JS Song, MS Ahn, BY Park, YI Lim, DS Han, YS 2024-01-21T08:39:07Z 2024-01-21T08:39:07Z 2021-09-03 2003-06-13 0006-291X https://pubs.kist.re.kr/handle/201004/138466 Hepatitis B virus (HBV) polymerase (Pol) interacts with cellular chaperone proteins and thereby performs multiple functions necessary for viral replication. Yeast two-hybrid analysis was applied to identify additional cellular targets required for HBV Pol function. HBV Pol interacted with S100A10 (p11), a Ca2+-modulated protein previously shown to bind to annexin II. The interaction between HBV Pol and p11 was confirmed by co-immunoprecipitation of the two proteins synthesized either in vitro or in transfected cells and by inhibition of the DNA polymerase activity of HBV Pol by p11. Immunofluorescence analysis of transfected human cell lines revealed that, although most HBV Pol and p11 was restricted to the cytoplasm, a small proportion of each protein colocalized as nuclear speckles; HBV Pol was not detected in the nucleus in the absence of p11. The HBV Pol-p11 nuclear speckles coincided with nuclear bodies containing the promyelocytic leukemia protein PML. Furthermore, the association of HBV Pol-p11 with PML was increased by exposure of cells to EGTA and inhibited by valinomycin. These results suggest a role for p11 n modulation of HBV Pol function and implicate PML nuclear bodies and intracellular Ca2+ in viral replication. (C) 2003 Elsevier Science (USA). All rights reserved. English ACADEMIC PRESS INC ELSEVIER SCIENCE REVERSE-TRANSCRIPTASE BINDING PROTEIN DNA COMPLEX CELLS PHOSPHORYLATION REPLICATION EXPRESSION INTERFERON INFECTION Association of hepatitis B virus polymerase with promyelocytic leukemia nuclear bodies mediated by the S100 family protein p11 Article 10.1016/S0006-291X(03)00881-7 1 BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, v.305, no.4, pp.1049 - 1056 BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 305 4 1049 1056 scie scopus 000183578500039 2-s2.0-0037566653 Biochemistry & Molecular Biology Biophysics Biochemistry & Molecular Biology Biophysics Article REVERSE-TRANSCRIPTASE BINDING PROTEIN DNA COMPLEX CELLS PHOSPHORYLATION REPLICATION EXPRESSION INTERFERON INFECTION annexin II-binding protein hepatitis B virus polymerase PML nuclear bodies p11