Lee, W Shin, HS Hong, JE Pyo, H Kim, Y 2024-01-21T08:44:19Z 2024-01-21T08:44:19Z 2022-01-11 2003-05-20 0253-2964 https://pubs.kist.re.kr/handle/201004/138562 An analytical method the determination of benzo(a)pyrene (BaP) and its hydroxylated metabolites, 1-hydroxybenzo(a)pyrene (1-OHBaP), 3-hydroxybenzo(a)pyrene (3-OHBaP), benzo(a)pyrene-4,5-dihydrodiol (4,5-diolBaP) and benzo(a)pyrene-7,8-dihydrodiol (7,8-diolBaP), in rat urine and plasma has been developed by HPLC/FLD and GC/MS. The derivatization with alkyl iodide was employed to improve the resolution and the detection of two mono hydroxylated metabolites, 1-OHBaP and 3-OHBaP, in LC and GC. BaP and its four metabolites in spiked urine were successfully separated by gradient elution on reverse phase ODS C-18 column (4.6 mm I.D., 100 mm length, particle size 5 mum) using a binary mixture of MeOH/H2O (85/15, v/v) as mobile phase after ethylation at 90 degreesC for 10 min. The extraction recoveries of BaP and its metabolites in spiked samples with liquid-liquid extraction, which was better than solid phase extraction, were in the range of 90.3-101.6% in n-hexane for urine and 95.7-106.3% in acetone for plasma, respectively. The calibration curves has shown good linearity with the correlation coefficients (R-2) varying from 0.992 to 1.000 for urine and from 0.996 to 1.000 for plasma, respectively. The detection limits of all analytes were obtained in the range of 0.01 - 0.1 ng/mL for urine and 0.1-0.4 ng/mL for plasma, respectively. The metabolites of BaP were excreted as mono hydroxy and dihydrodiol forms after intraperitoneal injection of 20 mg/kg of BaP to rats. The total amounts of BaP and four metabolites excreted in dosed rat urine were 3.79 ng over the 0-96 hr period from adminstration and the excretional recovery was less than 0.065% of the injection amounts of BaP. The proposed method was successfully applied to the determination of BaP and its hydroxylated metabolites in rat urine and plasma for the pharmacokinetic studies. English KOREAN CHEMICAL SOC POLYCYCLIC AROMATIC-HYDROCARBONS MASS-SPECTROMETRY BENZO<A>PYRENE METABOLITES PARTICULATE MATTER URINARY-EXCRETION RATS 1-HYDROXYPYRENE EXPOSURE Studies on the analysis of benzo(a)pyrene and its metabolites in biological samples by using high performance liquid chromatograpy/fluorescence detection and gas chromatography/mass spectrometry Article 1 BULLETIN OF THE KOREAN CHEMICAL SOCIETY, v.24, no.5, pp.559 - 565 BULLETIN OF THE KOREAN CHEMICAL SOCIETY 24 5 559 565 scie scopus kci ART000919891 000183819200010 2-s2.0-0041591133 Chemistry, Multidisciplinary Chemistry Article POLYCYCLIC AROMATIC-HYDROCARBONS MASS-SPECTROMETRY BENZO<A>PYRENE METABOLITES PARTICULATE MATTER URINARY-EXCRETION RATS 1-HYDROXYPYRENE EXPOSURE benzo(a)pyrene hydroxylated metabolites rat urine blood plasma HPLC/FLD