Kwon, OS Park, YJ Ryu, JC Chung, YB 2024-01-21T09:02:45Z 2024-01-21T09:02:45Z 2021-09-03 2003-05 0253-6269 https://pubs.kist.re.kr/handle/201004/138619 The analytical method of antispasmodic agent tiropramide {(+/-)alpha-(benzoylamino)-4-[2-(diethylamino)ethoxy]-N,N-dipropylbenzenepropanamide hydrochloride) was developed by gas chromatography/nitrogen-phosphorus detector (GC/NPD) in human plasma. Two kinds of tiropramide tablets were orally administered to volunteers by Latin square crossover design, and blood was withdrawn as designed schedule. The plasma of 1 mL was loaded on Sep-pak C-18 cartridge and eluted with methanol after washing with 30% methanol. The residue dissolved in 100 muL of methanol after evaporation was analyzed by GC/NPD. Precision (CV%) of intra-day was located within 2.6% and accuracy was less than 9.7%. Inter-day precision was below 8.7% and accuracy was relatively good as less than 14%. Plasma samples obtained from human volunteers were analyzed for the determination of tiropramide concentration by using this method. The method was sensitive, rapid and suitable enough to be applied for pharmacokinetic and bioequivalence studies of tiropramide in human volunteers. English PHARMACEUTICAL SOC KOREA Quantitative analysis of tiropramide in human blood by gas chromatography with nitrogen-phosphorus detector Article 10.1007/BF02976700 1 ARCHIVES OF PHARMACAL RESEARCH, v.26, no.5, pp.416 - 420 ARCHIVES OF PHARMACAL RESEARCH 26 5 416 420 N scie scopus kci ART001002466 000183186500011 2-s2.0-3242656974 Chemistry, Medicinal Pharmacology & Pharmacy Pharmacology & Pharmacy Article antispasmodic agent determination gas chromatography/nitrogen-phosphorus detector human plasma tiropramide