Cho, CH Lee, SY Shin, HS Philipson, KD Lee, CO 2024-01-21T09:03:30Z 2024-01-21T09:03:30Z 2021-09-03 2003-04-30 1226-3613 https://pubs.kist.re.kr/handle/201004/138632 The null mutation of cardiac Na+-Ca2+ exchanger (NCX1) gene in mice caused death of embryo in utero at embryonic day (ED) 9.0-9.5 and this embryonic lethality appears resulted from abnormal heart development. In the present study, we investigated whether transgenic re-expression of NCX1 in mutant cardiac myocytes could rescue these lethal defects. Transgenic mice expressing the canine NCX1 in a cardiac specific manner were bred into the NCX1 knock-out background but did not prevent the fetal lethality associated with the NCX1 null allele. However, the NCX1 knock-out embryos with an NCX1 transgene survived with heart beatings until ED 10.5 which was one day longer than the survival of the NCX1 knock-out embryos (ED 9.5). At ED 10.5, however, the partially rescued NCX1 embryos might have succumbed to the lack of an organized vasculature in the yolk sacs. The placental labyrinth layer was reduced in size and largely avascular. The transgenic re-expression of NCX1 rescued heart beatings and survived longer, but was still insufficient for the mice to be completely rescued. Importantly, NCX1 was observed to express in the yolk sac and the placenta of wild type mice. The results suggest that defects in extra-embryonic compartments are causal to the lethality, and that. NCX1 may play an important role in establishing vascularization in extra-embryonic tissues. English NATURE PUBLISHING GROUP SODIUM-CALCIUM EXCHANGER EMBRYONIC HEART DEVELOPMENT NA+/CA2+ EXCHANGER GENE-EXPRESSION MICE LACKING YOLK-SAC IMPLANTATION MECHANISMS APOPTOSIS ISOFORMS Partial rescue of the Na+-Ca2+ exchanger (NCX1) knock-out mouse by transgenic expression of NCX1 Article 10.1038/emm.2003.18 1 EXPERIMENTAL AND MOLECULAR MEDICINE, v.35, no.2, pp.125 - 135 EXPERIMENTAL AND MOLECULAR MEDICINE 35 2 125 135 scie scopus kci ART001189268 000183090100010 2-s2.0-0038823690 Biochemistry & Molecular Biology Medicine, Research & Experimental Biochemistry & Molecular Biology Research & Experimental Medicine Article SODIUM-CALCIUM EXCHANGER EMBRYONIC HEART DEVELOPMENT NA+/CA2+ EXCHANGER GENE-EXPRESSION MICE LACKING YOLK-SAC IMPLANTATION MECHANISMS APOPTOSIS ISOFORMS antiporters apoptosis blood vessels development embryo and fetal gene targeting