Paik, SY Ra, KS Chang, IS Park, YC Park, HS Baik, HS Yun, JW Choi, JN 2024-01-21T09:10:26Z 2024-01-21T09:10:26Z 2022-01-25 2003-03 1225-8687 https://pubs.kist.re.kr/handle/201004/138751 Hot water-soluble crude polysaccharide (HCAP-0) that was obtained from the fruits of Capsicum annuum showed potent anti-complementary activity. The activity was unchanged by pronase digestion, but decreased by periodate oxidation. The HCAP-0 was fractionated by DEAE ion-exchange chromatography to give two major fractions, HCAP-II and III. These two fractions were finally purified by gel filtration to give HCAP-IIa, HCAP-IIIa1, and IIIa2 fractions that had high anticomplementary activities. The HCAP-IIIa1 and IIIa2 consisted of homogeneous polysaccharides. The anticomplementary activities were unaffected by treatment with polymyxin B, indicating that the modes of complement activation were not due to preexisting lipopolysaccharide. The molecular weight and sugar content of HCAP-IIIa2 had potent anti-complementary activity. The highest yields were 55 kDa and 75.9%, and the molar ratio of galactose (Ara:Gal, 1.0:4.6) was higher than other sugars. The crossed immuno-electrophoresis showed that both classical and alternative pathways were activated by HCAP-IIIa2. English SPRINGER-VERLAG SINGAPORE PTE LTD Purification and characterization of complement-activating acidic polysaccharides from the fruits of Capsicum annuum Article 1 JOURNAL OF BIOCHEMISTRY AND MOLECULAR BIOLOGY, v.36, no.2, pp.230 - 236 JOURNAL OF BIOCHEMISTRY AND MOLECULAR BIOLOGY 36 2 230 236 N scie scopus kci ART000887685 000181925200014 2-s2.0-23944491176 Biochemistry & Molecular Biology Biochemistry & Molecular Biology Article ANGELICA-ACUTILOBA KITAGAWA BUPLEURUM-FALCATUM STRUCTURAL CHARACTERIZATION ROOTS ARABINOGALACTAN CAPSAICIN FRACTIONS EXTRACT BODIES acidic polysaccharide Capsicum annuum complement