Kim, Y Hwang, J Kim, M Lee, W 2024-01-21T09:32:57Z 2024-01-21T09:32:57Z 2021-09-01 2003-02 0951-4198 https://pubs.kist.re.kr/handle/201004/138897 Tripamide is a drug widely used in clinical practice for the treatment of hypertension and edema. This work evaluated a screening method for Tripamide and its urinary metabolites in human urine, using high-performance liquid chromatography diode-array detection (HPLC/DAD). Identification of these metabolites was investigated by high-performance liquid chromatography/electrospray ionization tandem mass spectrometry (HPLC/ESI-MS/MS) after dosing with 15 mg Tripamide. Acid hydrolysis showed that Tripamide is conjugated in the body. Two suspected metabolites were detected by HPLC/DAD. HPLC/ESI-MS/MS analysis suggested that these metabolites were probably hydroxylated together with loss of the -NH2 group and dehydrogenation. These results will be useful in confirmation methods for Tripamide in doping control. Copyright (C) 2003 John Wiley Sons, Ltd. English JOHN WILEY & SONS LTD PLASMA METABOLITES FUROSEMIDE Determination of Tripamide in human urine by high-performance liquid chromatography and high-performance liquid chromatography/electrospray ionization tandem mass spectrometry Article 10.1002/rcm.913 1 RAPID COMMUNICATIONS IN MASS SPECTROMETRY, v.17, no.4, pp.301 - 306 RAPID COMMUNICATIONS IN MASS SPECTROMETRY 17 4 301 306 scie scopus 000180952800006 2-s2.0-0037278891 Biochemical Research Methods Chemistry, Analytical Spectroscopy Biochemistry & Molecular Biology Chemistry Spectroscopy Article PLASMA METABOLITES FUROSEMIDE