Myung, SW Kim, HY Min, HK Kim, DH Kim, M Cho, HW Lee, HS Kim, JK Hong, CI 2024-01-21T09:44:25Z 2024-01-21T09:44:25Z 2021-09-04 2002-11 0951-4198 https://pubs.kist.re.kr/handle/201004/139098 The structural elucidation of fourteen metabolites of CKD-732, formed in vitro with rat liver microsomes, was performed using high-performance liquid chromatography/electrospray ionization-tandem mass spectrometry (HPLC/ESI-MS/MS). To identify proposed structures of the metabolites, the product ion mass spectra of the protonated molecules ([M + H](+)), the retention times on reversed-phase HPLC, and UV-Vis spectra were utilized. Characteristic product ions for the identification of CKD-732 metabolites were observed at m/z 231, 236, and 252. The fragment ions at m/z 236 and 252 indicated the unchanged form and the N-oxide of the dimethylamino-ethoxycinnamoyl group, respectively. The ion at m/z 231 indicated the presence of the hydroxylated form of the fumagillol group. The N-oxide of CKD-732, which was detected at m/z 515 and eluted later than CKD-732 in the reversed-phase HPLC system, was measured as a major metabolite. Three cis-trans isomers were also found. Copyright (C) 2002 John Wiley Sons, Ltd. English JOHN WILEY & SONS LTD CULTURED-HEPATOCYTES TNP-470 PERFORMANCE PLASMA TNP-470-AGM-1470 MICROSOMES ASSAY The identification of in vitro metabolites of CKD-732 by liquid chromatography/tandem mass spectrometry Article 10.1002/rcm.830 1 RAPID COMMUNICATIONS IN MASS SPECTROMETRY, v.16, no.21, pp.2048 - 2053 RAPID COMMUNICATIONS IN MASS SPECTROMETRY 16 21 2048 2053 scie scopus 000178955900007 2-s2.0-0036035798 Biochemical Research Methods Chemistry, Analytical Spectroscopy Biochemistry & Molecular Biology Chemistry Spectroscopy Article CULTURED-HEPATOCYTES TNP-470 PERFORMANCE PLASMA TNP-470-AGM-1470 MICROSOMES ASSAY CKD-732 metabolite LC/MS/MS