Oh, H Pae, HO Oh, GS Lee, SY Chai, KY Song, CE Kwon, TO Chung, HT Lee, HS 2024-01-21T10:13:53Z 2024-01-21T10:13:53Z 2021-09-01 2002-08 0032-0943 https://pubs.kist.re.kr/handle/201004/139360 Catalposide (1) and two related iridoids were isolated from the stem of Catalpa ovata (Bignoniaceae) by bioassay guided fractionation. Catalposide (1) significantly inhibited the production of nitric oxide (NO) in lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages in a dose-dependent manner. RT-PCR and Western blot analyses demonstrated that catalposide (1) suppressed the expression of inducible nitric oxide synthase (iNOS) gene and iNOS protein. Catalposide (1) also inhibited the activation of LPS-induced NF-kappaB as analyzed by electrophoretic mobility shift assay (EMSA). In addition to the inhibitory effect on NO production in LPS-stimulated RAW 264.7 cells, catalposide (1) significantly inhibited the NO production in cytokine-stimulated human DLD-1 and rat vascular smooth muscle (VSM) cells in a dose-dependent manner. English GEORG THIEME VERLAG KG IRIDOID GLYCOSIDE TRANSCRIPTION SYNTHASE Inhibition of inducible nitric oxide synthesis by catalposide from Catalpa ovata Article 10.1055/s-2002-33810 1 PLANTA MEDICA, v.68, no.8, pp.685 - 689 PLANTA MEDICA 68 8 685 689 scie scopus 000177914300005 2-s2.0-0036675486 Plant Sciences Chemistry, Medicinal Integrative & Complementary Medicine Pharmacology & Pharmacy Plant Sciences Pharmacology & Pharmacy Integrative & Complementary Medicine Article IRIDOID GLYCOSIDE TRANSCRIPTION SYNTHASE Catalpa ovata Bignoniaceae catalposide iNOS NF-kappa B