An, SJ Pae, HO Oh, GS Choi, BM Jeong, S Jang, SI Oh, H Kwon, TO Song, CE Chung, HT 2024-01-21T10:31:52Z 2024-01-21T10:31:52Z 2021-09-01 2002-07 1567-5769 https://pubs.kist.re.kr/handle/201004/139417 Catalposide, the major iridoid glycoside isolated from the stem bark of Catalpa ovata G. Don (Bignoniaceae), was found to inhibit the productions of tumor necrosis factor-alpha (TNF-alpha), interleukin-1beta (IL-1beta), and interleukin-6 (IL-6), and the activation of nuclear factor kappaB (NF-kappaB) in RAW 264.7 macrophages activated with lipopolysaccharide (LPS). Catalposide also inhibited the expressions of TNF-alpha, IL-1beta, and IL-6 genes and the nuclear translocation of p65 subunit of NF-kappaB in LPS-activated RAW 264.7 cells. Flow cytometric analysis revealed that catalposide suppressed the binding of FITC-conjugated LPS to CD 14 on the surface of cells, probably resulting in the inhibitory effects on TNT-alpha, IL- 1beta, and IL-6 productions and NF-kappaB activation. These findings suggest that catalposide could be an attractive candidate for adjunctive therapy in Gram-negative bacterial infections. (C) 2002 Elsevier Science B.V. All rights reserved. English ELSEVIER SCIENCE BV ENDOTOXIN-SHOCK MOLECULAR MECHANISM SEVERE SEPSIS SEPTIC SHOCK HYPERSENSITIVITY PATHOPHYSIOLOGY CHROMATOGRAPHY CYTOKINES SYSTEM CD14 Inhibition of TNF-alpha, IL-1 beta, and IL-6 productions and NF-kappa B activation in lipopolysaccharide-activated RAW 264.7 macrophages by catalposide, an iridoid glycoside isolated ftom Catalpa ovata G. Don (Bignoniaceae) Article 10.1016/S1567-5769(02)00085-1 1 INTERNATIONAL IMMUNOPHARMACOLOGY, v.2, no.8, pp.1173 - 1181 INTERNATIONAL IMMUNOPHARMACOLOGY 2 8 1173 1181 scie scopus 000178179300014 2-s2.0-0035993221 Immunology Pharmacology & Pharmacy Immunology Pharmacology & Pharmacy Article ENDOTOXIN-SHOCK MOLECULAR MECHANISM SEVERE SEPSIS SEPTIC SHOCK HYPERSENSITIVITY PATHOPHYSIOLOGY CHROMATOGRAPHY CYTOKINES SYSTEM CD14 LPS TNF-alpha IL-1 beta IL-6 NF-kappa B catalposide Catalpa ovata