Chen, W Han, DK Ahn, KD Kim, JM 2024-01-21T10:45:54Z 2024-01-21T10:45:54Z 2021-09-05 2002-04 1598-5032 https://pubs.kist.re.kr/handle/201004/139667 A molecularly imprinted polymer (MIP) having both amidine and imidazole functional groups in the active site has been prepared using p-nitrophenyl phosphate as a transition state analogue (TSA). The imprinted polymer MIP with amidine and imidazole found to have the highest hydrolysis activity compared with other MIPs with either amidine or imidazole groups only. It is postulated a cooperative effect between amidine and imidazole in the hydrolysis of p-nitrophenyl methyl carbonate (NPMC) as a substrate when both groups were arranged in proximity by molecular imprinting. The rate enhancement of the hydrolysis by MIP was 60 folds over the uncatalyzed solution reaction and two folds compared with the control non-imprinted polymer CP1 having both functional groups. The enzyme-mimetic catalytic hydrolysis of p-nitrophenyl acetate by MIP was evaluated in buffer at pH 7.0 with K-m of 1.06 mM and k(cat) of 0.137 h(-1). English POLYMER SOC KOREA BINDING Molecularly imprinted polymers having amidine and imidazole functional groups as an enzyme-mimetic catalyst for ester Hydrolysis Article 10.1007/BF03218301 1 MACROMOLECULAR RESEARCH, v.10, no.2, pp.122 - 126 MACROMOLECULAR RESEARCH 10 2 122 126 scie scopus kci 000175376000013 2-s2.0-0038699594 Polymer Science Polymer Science Article BINDING molecularly imprinted polymers molecularly imprinting catalysts enzyme-mimetic reaction amidine functional imidazole functional transition state analogue imprinting