Seo, JY Seong, SY Ahn, BY Kwon, IC Chung, H Jeong, SY 2024-01-21T11:04:46Z 2024-01-21T11:04:46Z 2021-09-05 2002-03 0019-9567 https://pubs.kist.re.kr/handle/201004/139741 The global use of a capsular polysaccharide-based pneumococcal vaccine has been limited because of serotype-specific protection and poor effectiveness in individuals with low immunocompetency. The mucosal immune system develops earlier in infants and lasts longer in the elderly than does the systemic immune system. Furthermore, mucosal immunization is beneficial for AIDS patients, because human immunodeficiency virus-infected subjects can develop normal mucosal antibody responses even in late clinical phases. For these reasons, we evaluated recombinant pneumococcal surface adhesin A (rPsaA) of Streptococcus pneumoniae in terms of cross-protective immune responses after oral delivery. Encapsulated rPsaA provided higher immunogenicity than naked rPsaA. Coencapsulation or codelivery of the cholera toxin (CT) B subunit (CTB) and CT also increased the immunogenicity of rPsaA. Cross-protective immunities against five strains of S. pneumoniae (types 4, 6B, 14, 19F, and 23F) were induced after oral immunization with microencapsulated rPsaA. Lung colonization and septicemia caused by the five serotypes were significantly inhibited by oral immunization with microencapsulated rPsaA. These results suggest that rPsaA coencapsulated with CTB can be used as an oral vaccine to induce cross-protective immunity for the prevention of pneumococcal infection. English AMER SOC MICROBIOLOGY CHOLERA-TOXIN B-SUBUNIT MUCOSAL PNEUMOLYSIN CHALLENGE VIRUS PSAA VIRULENCE PROTEINS ANTIBODY Cross-protective immunity of mice induced by oral immunization with pneumococcal surface adhesin A encapsulated in microspheres Article 10.1128/IAI.70.3.1143-1149.2002 1 INFECTION AND IMMUNITY, v.70, no.3, pp.1143 - 1149 INFECTION AND IMMUNITY 70 3 1143 1149 scie scopus 000173883100015 2-s2.0-0036176042 Immunology Infectious Diseases Immunology Infectious Diseases Article CHOLERA-TOXIN B-SUBUNIT MUCOSAL PNEUMOLYSIN CHALLENGE VIRUS PSAA VIRULENCE PROTEINS ANTIBODY microspheres