Kim, HJ Park, HS Hyun, MS Chang, IS Kim, M Kim, BH 2024-01-21T11:06:24Z 2024-01-21T11:06:24Z 2021-09-04 2002-02-14 0141-0229 https://pubs.kist.re.kr/handle/201004/139771 Direct electron transfer from different Shewanella putrefaciens strains to an electrode was examined using cyclic voltammetry and a fuel cell type electrochemical cell. Both methods determine the electrochemical activity of the bacterium without any electrochemical mediators. In the cyclic voltammetric studies, anaerobically grown cells of Shewanella putrefaciens MR-1, IR-1, and SR-21 showed electrochemical activities, but no activities were observed in aerobically grown Shewanella putrefaciens cells nor in aerobically and anaerobically grown E. coli cell suspensions. The electrochemical activities measured by the cyclic voltammetric method were closely related to the electric potential and current generation capacities in the microbial fuel cell system. Cytochromes localized to the outer membrane are believed to facilitate the direct electron transfer to the electrode from the intact bacterial cells. The concentration of the electron donor in the anode compartment determined the current generation capacity and potential development in the microbial fuel cell. When the high concentration of the bacteria (0.47 g dry cell weight/liter) and an electrode that has large surface area (apparent area: 50 cm(2)) were used, relatively high Coulombic yield (over 3 C for 12 h) was obtained from the bacteria. (C) 2002 Elsevier Science Inc. All rights reserved. English ELSEVIER SCIENCE INC C-TYPE CYTOCHROME FE(III)-REDUCING BACTERIUM OUTER-MEMBRANE SP-NOV DISSIMILATORY FE(III) ELECTRON-TRANSFER MN(IV) REDUCTION FERRIC IRON GEN-NOV MICROORGANISM A mediator-less microbial fuel cell using a metal reducing bacterium, Shewanella putrefaciense Article 10.1016/S0141-0229(01)00478-1 1 ENZYME AND MICROBIAL TECHNOLOGY, v.30, no.2, pp.145 - 152 ENZYME AND MICROBIAL TECHNOLOGY 30 2 145 152 scie scopus 000173917900002 2-s2.0-0037074898 Biotechnology & Applied Microbiology Biotechnology & Applied Microbiology Article C-TYPE CYTOCHROME FE(III)-REDUCING BACTERIUM OUTER-MEMBRANE SP-NOV DISSIMILATORY FE(III) ELECTRON-TRANSFER MN(IV) REDUCTION FERRIC IRON GEN-NOV MICROORGANISM mediator-less microbial fuel cell Shewanella purefaciens metal-reducing bacteria