Rhim, H Kim, H Lee, DY Oh, TH Nah, SY 2024-01-21T11:06:30Z 2024-01-21T11:06:30Z 2021-09-05 2002-02-02 0014-2999 https://pubs.kist.re.kr/handle/201004/139773 There is increasing evidence that ginseng influences pain modulation. In spite of extensive behavior studies, the detailed mechanism of ginseng actions at the cellular level and the identity of the active substance have not been elucidated yet. Whole-cell patch-clamp recordings were used to examine the modulation of high-voltage-activated Ca2+ channel currents by ginseng total saponins and its various individual ginsenosides in rat dorsal root ganglion neurons. Application of ginseng total saponins suppressed Ca2+ channel currents in a dose-dependent manner. Occlusion experiments using selective blockers revealed that ginseng total saponins could modulate L-, N-, and P-type currents. The co-application of ginseng total saponins and the p-opioid receptor agonist, D-Ala(2), N-MePhe(4), Gly(5)-ol-enkephalin (DAMGO), produced non-additive effects in most cells tested and each effect was significantly relieved by a depolarizing prepulse. Overnight treatment of cells with pertussis toxin profoundly reduced the inhibition. Furthermore, we now report that ginsenoside Rg(3), among the major fractions of ginseng saponins, is a newly identified active component for the inhibition. These results suggest that the modulation of Ca2+ channels by ginseng total saponins, in particular by ginsenoside Rg(3), could be part of the pharmacological basis of ginseng-mediated antinociception. (C) 2002 Elsevier Science B.V. All rights reserved. English ELSEVIER SCIENCE BV NUCLEUS-TRACTUS-SOLITARIUS CALCIUM CHANNELS PHARMACOLOGICAL ACTIONS PANAX-GINSENG ROOT INHIBITION RECEPTOR MORPHINE EXTRACT ANTINOCICEPTION Ginseng and ginsenoside Rg(3), a newly identified active ingredient of ginseng, modulate Ca2+ channel currents in rat sensory neurons Article 10.1016/S0014-2999(01)01613-2 1 EUROPEAN JOURNAL OF PHARMACOLOGY, v.436, no.3, pp.151 - 158 EUROPEAN JOURNAL OF PHARMACOLOGY 436 3 151 158 scie scopus 000174129600001 2-s2.0-0037006173 Pharmacology & Pharmacy Pharmacology & Pharmacy Article NUCLEUS-TRACTUS-SOLITARIUS CALCIUM CHANNELS PHARMACOLOGICAL ACTIONS PANAX-GINSENG ROOT INHIBITION RECEPTOR MORPHINE EXTRACT ANTINOCICEPTION ginseng ginsenoside antinociception patch-clamp Ca2+ channel current pertussis toxin sensory neurons