Sang, Jun Sim Snell, KD Kim, BW Rha, CK Sinskey, AJ 2024-01-21T11:15:02Z 2024-01-21T11:15:02Z 2022-04-05 2001-12 0141-5492 https://pubs.kist.re.kr/handle/201004/139923 A second promoter (P1) was inserted to the PHA (poly-beta-hydroxyalkanoate) operon (pSP2) of Esherichia coli DH5alpha with an optimal E. coli ribosome binding site and a trc strong promoter (pSJS1) to obtain poly-beta-hydroxybutyrate (PHB) with long chain length. When the inducer, IPTG was added to the culture at 0.4 mM, the average molecular weight was 1.1 x 10(6) Da. However, an even greater increase of the PHB average molecular weight to 2.5 x 10(7) Da was observed without IPTG being added. English Kluwer Academic Publishers Increased poly-beta-hydroxybutyrate (PHB) chain length by the modulation of PHA synthase activity in recombinant Escherichia coli Article 10.1023/A:1013752000022 1 Biotechnology Letters, v.23, no.24, pp.2057 - 2061 Biotechnology Letters 23 24 2057 2061 N scopus 000173206600014 2-s2.0-0035693242 Biotechnology & Applied Microbiology Biotechnology & Applied Microbiology Article POLYHYDROXYALKANOATES POLYHYDROXYBUTYRATE BACTERIAL PLANTS average molecular weight PHA synthase polyhydroxybutyrate