Park, HS Kim, BH Kim, HS Kim, HJ Kim, GT Kim, M Chang, IS Park, YK Chang, HI 2024-01-21T11:33:20Z 2024-01-21T11:33:20Z 2021-09-05 2001-12 1075-9964 https://pubs.kist.re.kr/handle/201004/139987 An obligatory anaerobic bacterium was isolated from a mediator-less microbial fuel cell using starch processing wastewater as the fuel and designated as EG3. The isolate was Gram-positive, motile and rod (2.8-3.0 mum long, 0.5-0.6 mum wide). The partial 16S rRNA gene sequence and analysis of the cellular fatty acids profile suggested that EG3 clusters with Clostridium sub-phylum and exhibited the highest similarity (98%) with Clostridium butyricum. The temperature and pH optimum for growth were 37degreesC and 7.0, respectively. The major products of glucose and glucose/Fe(O)OH metabolism were lactate, formate, butyrate, acetate, CO2 and H-2. Growth was faster at the initial phase and the cell yield was higher when the medium was supplemented with Fe(O)OH than without Fe(O)OH. These results suggest that Fe(III) ion is utilised as an electron sink. Cyclic voltammetry showed that Clostridium butyricum EG3 cells were electrochemically active. It is a novel characteristic of strict anaerobic Gram-positive bacteria. (C) 2001 Academic Press. English ACADEMIC PRESS LTD ELSEVIER SCIENCE LTD SHEWANELLA-PUTREFACIENS ELECTRODE-REACTION GEN-NOV REDUCTION IRON FERMENTATION CAPSULATUS MANGANESE PHOSPHATE OXIDATION A novel electrochemically active and Fe(III)-reducing bacterium phylogenetically related to Clostridium butyricum isolated from a microbial fuel cell Article 10.1006/anae.2001.0399 1 ANAEROBE, v.7, no.6, pp.297 - 306 ANAEROBE 7 6 297 306 scie scopus 000173983800003 2-s2.0-0035717337 Microbiology Microbiology Article SHEWANELLA-PUTREFACIENS ELECTRODE-REACTION GEN-NOV REDUCTION IRON FERMENTATION CAPSULATUS MANGANESE PHOSPHATE OXIDATION Clostridium sp EG3 dissimilatory Fe(III) reduction cyclic voltammogram electrochemically active bacteria microbial fuel cell