Choi, MH Kim, KR Kim, DH Chung, BC 2024-01-21T11:33:23Z 2024-01-21T11:33:23Z 2021-09-05 2001-12 0009-5893 https://pubs.kist.re.kr/handle/201004/139988 Medroxyprogesterone acetate (MPA) is one of the major drugs used in endocrine therapy for advanced or recurrent breast cancer. The present study describes an accurate and reproducible gas chromatographic-mass spectrometric (GC-MS) method to measure MPA in serum. After being extracted by a solid-phase cartridge using an Oasis HLB copolymer, the residues were derivatized with a heptafluorobutyric anhydride and then analyzed by GC-MS. We validated the method by analyzing the serum samples spiked with pure MPA, and obtained a linear correlation (0.9977) and precision with a coefficient of variation ranging from 1.8 to 73. We found that the GC-MS method was linear over the range of 0.2 ng mL(-1) to 500 ng mL(-1) using a sample volume of 1 mL The analytical recovery was 93.7%, and the limit of detection was 0.2 ng mL(-1). The oral administration of MPA to men and its subsequent detection in serum showed that the GC-MS method described is sensitive, specific, and suitable for the determination of MPA by bench-top GC-MS. English VIEWEG ANABOLIC-STEROIDS DERIVATIZATION PLASMA URINE Rapid and sensitive analysis of medroxyprogesterone acetate in human serum by gas chromatography-mass spectrometry Article 10.1007/BF02492496 1 CHROMATOGRAPHIA, v.54, no.11-12, pp.765 - 769 CHROMATOGRAPHIA 54 11-12 765 769 scie scopus 000172936700012 2-s2.0-0035660283 Biochemical Research Methods Chemistry, Analytical Biochemistry & Molecular Biology Chemistry Article ANABOLIC-STEROIDS DERIVATIZATION PLASMA URINE gas chromatography-mass spectrometry solid phase extraction medroxyprogesterone acetate human serum samples