Jung, BH Moon, D Chung, BC 2024-01-21T11:33:43Z 2024-01-21T11:33:43Z 2021-09-05 2001-12 1205-6685 https://pubs.kist.re.kr/handle/201004/139994 A simple and reliable high performance liquid chromatographic (HPLC) assay method was developed for the simultaneous determination of sildenafil and its demethylated metabolite (UK-103,320) in cat plasma. Solid-phase extraction with a C-18 SepPak cartridge after deproteinization was used, and the extraction recoveries were over 85% in both sildenafil and UK103,320. The coefficient of variation was less than 15% in the 50-1000 ng/ml range for both sildenafil and UK-103,320, indicating that this assay has adequate reproducibility. This method was used to perform pharmacokinetic studies following oral administration of sildenafil citrate. Moment analysis revealed that the elimination half-life (7.4+/-2.1 hr) was similar to those reported in studies using [C-14]-sildenafil and the AUC percentage of UK-103,320 to sildenafil was also similar to the results of a previous report. These results indicate that this convenient analytical method is readily applicable to pharmacokinetic studies of sildenafil and UK-103,320. English SPECTROSCOPY SOC CANADA, OTTAWA MALE ERECTILE DYSFUNCTION VIAGRA HPLC determination of sildenafil and its demethylated metabolite, UK-103,320, in cat plasma with solid-phase extraction for the pharmacokinetic study Article 1 CANADIAN JOURNAL OF ANALYTICAL SCIENCES AND SPECTROSCOPY, v.46, no.2, pp.40 - 45 CANADIAN JOURNAL OF ANALYTICAL SCIENCES AND SPECTROSCOPY 46 2 40 45 scie scopus 000173220300001 2-s2.0-0035647871 Chemistry, Analytical Spectroscopy Chemistry Spectroscopy Article MALE ERECTILE DYSFUNCTION VIAGRA sildenafil UK-103,320 HPLC solid phase extraction pharmacokinetic