Jung, BH Chang, BS Chung, BC 2024-01-21T11:39:19Z 2024-01-21T11:39:19Z 2021-09-04 2001-11 0003-2719 https://pubs.kist.re.kr/handle/201004/140095 A simple and reliable, high-performance liquid chromatographic (HPLC) method for the determination of vitamin E in porcine plasma with UV detection is described. Liquid-liquid extraction with ether was used, and the extraction recovery was improved to 82% by adding NaCl to the aqueous phase. The mobile phase was comprised of methanol and water. and a gradient system. was used. in which the percentage of methanol was increased from 70% to 100%. The flow rate was 1.5 ml/min, and the peaks were monitored at 220 nm in a UV detector. The detection limit was 1 mug/ml, and the coefficient of variation was less than 10% in the 3-15 mug/ml range and less than 12.3% in the 1 mug/ml. This method was adapted to the analysis of porcine plasma obtained after oral administration of vitamin E-enriched feedstuffs. After the analysis, it was apparent that the plasma level was maintained within 1-5 mug/ml in all of the pigs. English MARCEL DEKKER INC ALPHA-TOCOPHEROL BETA-CAROTENE RETINOL INFANTS Determination of vitamin E in porcine plasma after oral administration of vitamin E-enriched feedstuffs using high performance liquid chromatography Article 10.1081/AL-100107296 1 ANALYTICAL LETTERS, v.34, no.13, pp.2303 - 2310 ANALYTICAL LETTERS 34 13 2303 2310 scie scopus 000171926200007 2-s2.0-0034754809 Chemistry, Analytical Chemistry Article ALPHA-TOCOPHEROL BETA-CAROTENE RETINOL INFANTS vitamin E HPLC salting out effect porcine plasma