Namkung, Y Skrypnyk, N Jeong, MJ Lee, T Lee, MS Kim, HL Chin, H Suh, PG Kim, SS Shin, HS 2024-01-21T11:43:17Z 2024-01-21T11:43:17Z 2021-09-05 2001-10 0021-9738 https://pubs.kist.re.kr/handle/201004/140164 Pancreatic 5 cells are the source of insulin, which directly lowers blood glucose levels in the body. Our analyses Of alpha (1D) gene-knockout (alpha (1D-/-)) mice show that the L-type calcium channel, alpha (1D), is required for proper beta cell generation in the postnatal pancreas. Knockout mice were characteristically slightly smaller than their littermates and exhibited hypoinsulinemia and glucose intolerance. However, isolated a(1D)(-/-) islets persisted in glucose sensing and insulin secretion, with compensatory overexpression of another L-type channel gene, alpha (1C). Histologically, newborn a(1D)(-/-) mice had an equivalent number of islets to wild-type mice. in contrast, adult alpha (-/-)(1D) mice showed a decrease in the number and size of islets, compared with littermate wild-type mice due to a decrease in beta cell generation. TUNEL staining showed that there was no increase in cell. death in alpha (-/-)(1D) islets, and a 5-bromo-2 ' deoxyuridine-labeling (BrdU-labeling) assay illustrated significant reduction in the proliferation rate of beta cells in alpha (-/-)(1D) islets. English AMER SOC CLINICAL INVESTIGATION INC Requirement for the L-type Ca2+ channel alpha(1D) subunit in postnatal pancreatic beta cell generation Article 10.1172/JCI13310 1 JOURNAL OF CLINICAL INVESTIGATION, v.108, no.7, pp.1015 - 1022 JOURNAL OF CLINICAL INVESTIGATION 108 7 1015 1022 N scie scopus 000171341400010 2-s2.0-0034794289 Medicine, Research & Experimental Research & Experimental Medicine Article INCREASED INSULIN SENSITIVITY DEPENDENT CALCIUM-CHANNEL ACTIVIN RECEPTOR FUNCTIONAL EXPRESSION DIABETES-MELLITUS TRANSGENIC MICE B-CELLS SECRETION GROWTH ISLETS