Baek, M Jeong, JH Kim, DH 2024-01-21T12:33:08Z 2024-01-21T12:33:08Z 2021-09-05 2001-04-15 0378-4347 https://pubs.kist.re.kr/handle/201004/140528 A column-switching high-performance liquid chromatography (HPLC) method for the determination of aloesin in rat plasma using column-switching and ultraviolet (UV) absorbance detection is described. Plasma was directly injected onto the HPLC system consisting of a clean-up column, a concentrating column, and an analytical column, which were connected with a six-port switching valve. The determination of aloesin was accurate and repeatable, with a limit of quantitation of 10 ng/ml in plasma. The standard calibration curve for aloesin was linear (r=0.998) over the concentration range of 10-1000 ng/ml in rat plasma. The intra- and inter-day assay variabilities of aloesin ranged from 1.0 to 4.7% and 1.1 to 8.8%, respectively. This highly sensitive and simple method has been successfully applied to a pharmacokinetic study after oral administration of aloesin to rats. (C) 2001 Elsevier Science B.V. All rights reserved. English ELSEVIER SCIENCE BV ARBORESCENS MILLER Determination of aloesin in rat plasma using a column-switching high-performance liquid chromatographic assay Article 10.1016/S0378-4347(00)00593-4 1 JOURNAL OF CHROMATOGRAPHY B, v.754, no.1, pp.121 - 126 JOURNAL OF CHROMATOGRAPHY B 754 1 121 126 scie scopus 000167900900014 2-s2.0-0035871939 Biochemical Research Methods Chemistry, Analytical Biochemistry & Molecular Biology Chemistry Article ARBORESCENS MILLER aloesin